α-突触核蛋白在食蟹猴消化道不同部位的表达

doi:10.3969/j.issn.1006-7795.2015.05.002

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (5): 675-679.doi: 10.3969/j.issn.1006-7795.2015.05.002

• PD的发病机制与治疗 • 上一篇下一篇

α-突触核蛋白在食蟹猴消化道不同部位的表达

李旭冉^1,2, 杨巍巍^1,2, 李昕^1,2, 于顺^1,2,3

1. 首都医科大学宣武医院神经生物学研究室, 北京 100053;
2. 帕金森病研究北京重点实验室, 北京 100053;
3. 北京脑重大疾病研究院帕金森病研究所, 北京 100053

收稿日期:2015-09-14 出版日期:2015-10-21 发布日期:2015-10-20
基金资助:
国家重点基础研究发展计划(2011CB504101),国家自然科学基金(81071014, 81371200,81401042),国家科技支撑计划课题(2012BAI10B03),北京市自然科学基金(7122035),首都卫生发展科研专项课题(2011-4001-01)。

Expression of α-synuclein in different regions of digestive tract of cynomolgus monkeys

Li Xuran^1,2, Yang Weiwei^1,2, Li Xin^1,2, Yu Shun^1,2,3

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China;
3. Center for Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing 100053, China

Received:2015-09-14 Online:2015-10-21 Published:2015-10-20
Contact: 于顺 E-mail:yushun103@163.com
Supported by:
This study was supported by Major State Basic Research Development Program of China(973 Program) (2011CB504101), National Natural Science Foundation of China (81071014, 81371200, 81401042), National Science and Technology Support Program (2012BAI10B03), Natural Science Foundation of Beijing (7122035), Capital Health Research and Development Special Fund (2011-4001-01).

摘要/Abstract

摘要： 目的观察α-突触核蛋白(α-synuclein, α-Syn)在正常食蟹猴消化道不同部位的表达。方法利用免疫组织化学和Western blotting方法检测α-Syn在食蟹猴食道、胃、十二指肠、空肠、回肠及结肠中的表达特点和表达量。结果 α-Syn免疫活性物质主要见于消化道壁内神经的神经纤维和神经节,这些α-Syn免疫阳性神经结构见于消化道的黏膜、黏膜下层和肌层。在消化道的不同部位,α-Syn的表达量不同,其中十二指肠、空肠、回肠和结肠表达量较高,而胃和食道的表达量较低。结论 α-Syn主要存在于食蟹猴消化道的壁内神经,在消化道的不同部位表达量差异有统计学意义。

关键词: α-突触核蛋白, 消化道, 食蟹猴, 壁内神经

Abstract: Objective To investigate the expression of α-synuclein (α-Syn) in different regions of the digestive tract of cynomolgus monkeys. Methods Immunohistochemistry and Western blotting analysis were applied to observe the expression of α-Syn in the digestive tract of cynomolgus monkeys. Results α-Syn immunoreactive substances were observed in both the nerve fibers and nerve plexus in the wall of the digestive tract of cynomolgus monkeys. The α-Syn immunopositive structures were found in the mucosa, submucosal nerve plexus, and myenteric nerve plexus of the digestive tract. The amounts of α-Syn in different regions of the digestive tract were different, with the higher level of α-Syn found in the duodenum, jejunum, ileum, and colon, and lower levels of stomach and esophagus. Conclusion α-Syn is mainly localized in the intrinsic nerves in the digestive tract of cynomolgus monkeys, with the levels differing in different regions of the digestive tract.

Key words: α-synuclein, digestive tract, cynomolgus monkeys, intrinsic nerve

中图分类号:

R338

李旭冉, 杨巍巍, 李昕, 于顺. α-突触核蛋白在食蟹猴消化道不同部位的表达[J]. 首都医科大学学报, 2015, 36(5): 675-679.

Li Xuran, Yang Weiwei, Li Xin, Yu Shun. Expression of α-synuclein in different regions of digestive tract of cynomolgus monkeys[J]. Journal of Capital Medical University, 2015, 36(5): 675-679.

参考文献

[1] Jellinger K A. Neuropathobiology of non-motor symptoms in parkinson disease[J]. J Neural Transm,2015, May 15. [Epub ahead of print].
[2] Braak H, Del Tredici K, Rüb U, et al. Staging of brain pathology related to sporadic Parkinson's disease[J]. Neurobiol Aging,2003, 24(2): 197-211.
[3] Holmqvist S, Chutna O, Bousset L, et al. Direct evidence of parkinson pathology spread from the gastrointestinal tract to the brain in rats[J]. Acta Neuropathol,2014, 128(6): 805-820.
[4] Yu S, Li X, Liu G, et al. Extensive nuclear localization of alpha-synuclein in normal rat brain neurons revealed by a novel monoclonal antibody [J]. Neuroscience,2007, 145(2): 539-555.
[5] Selkoe D, Dettmer U,Luth E, et al. Defining the native state of α-synuclein [J]. Neurodegener Dis,2014, 13(2-3): 114-117.
[6] Luth E S, Bartels T, Dettmer U, et al. Purification of α-synuclein from human brain reveals an instability of endogenous multimers as the protein approaches purity [J]. Biochemistry,2015, 54(2): 279-292.
[7] Ito S, Takao M, Hatsuta H, et al. Alpha-synuclein immunohistochemistry of gastrointestinal and biliary surgical specimens for diagnosis of lewy body disease[J]. Int Clin Exppathol, 2014,7(4):1714-1723.
[8] Chin-Chan M, Navarro-Yepes J, Quintanilla-Vega B. Environmental pollutants as risk factors for neurodegenerative disorders: alzheimer and parkinson diseases[J]. Front Cell Neurosci,2015, 9: 1-24.
[9] Natale G, Kastsiushenka O, Fulceri F, et al. MPTP-induced parkinsonism extends to a subclass of TH-positive neurons in the gut[J]. Brain Res,2010, 1355: 195-206.
[10] Chaudhuri K R, Yates L, Martinez-Martin P. The non-motor symptom complex of Parkinson's disease: a comprehensive assessment is essential[J]. Curr Neurol Neurosci Rep,2005, 5(4): 275-283.
[11] Abbott R D, Petrovitch H, White L R, et al. Frequency of bowel movements and the future risk of Parkinson's disease[J]. Neurology,2001, 57(3): 456-462.
[12] 李昕, 杨巍巍,李旭冉,等. 寡聚化α-突触核蛋白在不同年龄段食蟹猴消化道中的表达[J]. 首都医科大学学报,2014,35(5) :592-595.
[13] 尹娜, 陈予东,李昕,等. 帕金森病患者血浆增强α-突触核蛋白寡聚体形成[J]. 首都医科大学学报, 2013,34(6) :840-843.

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α-突触核蛋白在食蟹猴消化道不同部位的表达

Expression of α-synuclein in different regions of digestive tract of cynomolgus monkeys

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