基于厚朴酚对白色念珠菌黏附性及其生物膜形成的影响探讨其抗龋作用

doi:10.3969/j.issn.1006-7795.2015.06.019

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (6): 942-945.doi: 10.3969/j.issn.1006-7795.2015.06.019

基于厚朴酚对白色念珠菌黏附性及其生物膜形成的影响探讨其抗龋作用

向晓波¹, 周艳萌², 冯琳颖³, 董华⁴

1. 遵义医学院附属口腔医院口腔外科, 贵州遵义 563000;
2. 遵义医学院微生物与免疫学实验室, 贵州遵义 563000;
3. 遵义医学院医学与科技学院2011级, 贵州遵义 563000;
4. 遵义医学院医学与科技学院2012级, 贵州遵义 563000

收稿日期:2015-09-28 出版日期:2015-12-21 发布日期:2015-12-18
通讯作者: 向晓波 E-mail:307879470@qq.com
基金资助:
贵州省中医药管理局课题(ky2015-9)。

Based on the impact of magnolol on Candida albicans adhesion and biofilm formation for exploring its anti-caries effect

Xiang Xiaobo¹, Zhou Yanmeng², Feng Linying³, Dong Hua⁴

1. Department of Stomatological Surgery, Affiliated Stomatology Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China;
2. Department of Microbiology and Immunology, Zunyi Medical University, Zunyi 563000, Guizhou Province, China;
3. Grade 2011 of the Medicine & Technology School, Zunyi Medical University, Zunyi 563000, Guizhou Province, China;
4. Grade 2012 of the Medicine & Technology School, Zunyi Medical University, Zunyi 563000, Guizhou Province, China

Received:2015-09-28 Online:2015-12-21 Published:2015-12-18
Supported by:
This study was supported by Projects of Administration of Traditional Chinese Medicine by Guizhou Province(ky2015-9).

摘要/Abstract

摘要： 目的观察厚朴酚对白色念珠菌黏附性及生物膜形成的影响,以探讨其能否用于治疗白色念珠菌相关龋病。方法运用微孔板法复制白色念珠菌体外黏附及生物膜模型,采用XTT〔2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide〕减低法评价不同浓度厚朴酚对白色念珠菌黏附、生物膜形成的影响。结果厚朴酚对白色念珠菌的黏附具有抑制作用,其作用具有时间及浓度依赖性,62.5 mg/L和125 mg/L厚朴酚可以显著的抑制白色念珠菌的黏附,与阴性对照组相比,差异均有统计学意义(P < 0.05);实验所选5个浓度的厚朴酚对白色念珠菌的生物膜均有不同程度的抑制作用,其中125 mg/L厚朴酚对生物膜的抑制率达到66.32%。结论厚朴酚可通过抑制白色念珠菌的黏附而有效降低其致病性,并对已形成的生物膜具有明显的抑制作用,提示其作为抗龋药物有良好的应用前景。

关键词: 厚朴酚, 白色念珠菌, 龋病

Abstract: Objective To observe the impact of magnololon Candida albicans adhesion and biofilm formation in order to assess its anti-caries effect. Methods Through 96-well microtiter plate copy C.albicans in vitro adhesion and biofilm model, XTT reduction assay was applied to evaluate the effect of the different concentrations of magnolol on C.albicans adhesion and biofilm formation. Results Magnolol has inhibitory effect on C. albicans adhesion, its action has the time and the concentration dependence. The magnolol of 62.5 mg/L and 125 mg/L of magnolol had significantly inhibited adhesion of C. albicans, compared with the negative control group, the difference was statistically significant(P < 0.05). Test the concentration of the selected five magnolol had different degrees of inhibition on C. albicans biofilms, of which the inhibition rates of 125 mg/L magnolol against C. albicans biofilm was 66.32%. Conclusion Magnolol can inhibit adhesion of C. albicans and reduce its pathogenicity, and has obvious inhibitory effect to the formed biofilm. The results suggest magnolol has a good application prospect as an anti-caries drug.

Key words: magnolol, candida albicans, dental caries

中图分类号:

R988.2

向晓波, 周艳萌, 冯琳颖, 董华. 基于厚朴酚对白色念珠菌黏附性及其生物膜形成的影响探讨其抗龋作用[J]. 首都医科大学学报, 2015, 36(6): 942-945.

Xiang Xiaobo, Zhou Yanmeng, Feng Linying, Dong Hua. Based on the impact of magnolol on Candida albicans adhesion and biofilm formation for exploring its anti-caries effect[J]. Journal of Capital Medical University, 2015, 36(6): 942-945.

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基于厚朴酚对白色念珠菌黏附性及其生物膜形成的影响探讨其抗龋作用

Based on the impact of magnolol on Candida albicans adhesion and biofilm formation for exploring its anti-caries effect

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