NK细胞与早发型重度子痫前期疾病进展的相关性研究

doi:10.3969/j.issn.1006-7795.2016.04.025

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (4): 538-542.doi: 10.3969/j.issn.1006-7795.2016.04.025

NK细胞与早发型重度子痫前期疾病进展的相关性研究

赵秀伶¹, 马红梅¹, 马唯¹, 单志娟², 洛丽³

1. 首都医科大学附属北京潞河医院产科, 北京 101100;
2. 首都医科大学附属北京潞河医院检验科, 北京 101100;
3. 首都医科大学附属北京潞河医院病理科, 北京 101100

收稿日期:2016-03-01 出版日期:2016-08-21 发布日期:2016-07-18
通讯作者: 赵秀伶 E-mail:lhyyzxl@hotmail.com

Study about the correlation of NK cell with early onset preeclampsia

Zhao Xiuling¹, Ma Hongmei¹, Ma Wei¹, Shan Zhijuan², Luo Li³

1. Department of Obstetrics and Gynecology, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China;
2. Department of Clinical Laboratory, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China;
3. Department of Pathology, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China

Received:2016-03-01 Online:2016-08-21 Published:2016-07-18

摘要/Abstract

摘要： 目的探讨自然杀伤细胞（natural killer cell，NK）与早发型重度子痫前期发生、发展的相关关系。方法选取2012年1月至2014年12月在北京潞河医院定期孕检的重度子痫前期患者44例（早发型重度子痫前期20例，晚发型重度子痫前期24例）为研究对象。选取同时期正常无合并症的患者20例为对照组。采集母亲外周血2 mL，胎儿脐带血2 mL及蜕膜组织。采用流式细胞学方法检测外周血和脐带血中的CD3^-CD16^-CD56⁺的百分率；蜕膜组织应用免疫组织化学方法，计算CD3^-CD16^-CD56⁺的数量取均值，应用计算机图像分析法读取CD56⁺细胞平均灰度值。结果重度子痫前期组外周血、脐血、蜕膜组织中的NK细胞数量高于对照组，差异有统计学意义（均P<0.05）；早发型重度子痫前期组脐血、蜕膜组织中NK细胞数量高于晚发型重度子痫前期组，差异有统计学意义（P<0.05）。重度子痫前期外周血、蜕膜组织中的NK细胞数量与病程进展呈负相关（r=-0.397，P=0.040；r=-0.391，P=0.044），与血压升高幅度呈正相关（r=-0.597，P=0.001；r=-0.416，P=0.031）。蜕膜组织中的NK细胞数量与确诊重度子痫前期的孕周呈负相关（r=-0.594，P=0.003）。结论重度子痫前期患者外周血、脐血、蜕膜中NK细胞数量增加，早发型重度子痫前期增加更为显著。外周血、蜕膜中NK细胞数量与早发型重度子痫前期的发生、发展相关。

关键词: 早发型重度子痫前期, 自然杀伤细胞, 蜕膜

Abstract: Objective To study the correlation of natural killer cell (NK) to early onset preeclampsia. Methods This research adopts the case-control study. 44 preeclampsia patients who were regularly checked up in BeiJing LUHE hospital from Jan 2012 to Dec 2014 entered the research. The group included 20 early onset preeclampsia patients and 24 late onset preeclampsia patients. The control group was normal patients at the same period. Collecting maternal peripheral blood umbilical blood and deciduas. We test percentage of NK cell in peripheral and umbilical blood by Flow cytometry. NK cells in deciduas were tested by immunohistochemistry. Results The courts of NK cells in maternal peripheral, umbilical blood and deciduas of early onset preeclampsia and late onset preeclampsia were all higher than those of the normal (P<0.05). It is significantly different. The counts of the NK cells in umbilical blood and deciduas were significantly higher than those of the late onset preeclampsia patients (P<0.05). The NK cell percentage in maternal peripheral blood in early onset preeclampsia patients were higher than in late onset preeclampsia patients. P<0.05. The courts of NK cells in maternal peripheral and deciduas was with negative correlation with course of preeclampsia.(r=-0.397, P=0.040;r=-0.391, P=0.044). It was positive correlation with the degree of elevation of pressure. (r=-0.597, P=0.001;r=-0.416, P=0.031). The courts of NK cells in deciduas was positive correlation with the time of diagnosis. (r=-0.594, P=0.003). Conclusion The counts of the NK cell in maternal peripheral blood, umbilical blood and deciduas in preeclampsia patients were significantly higher than those of the control. Early onset severe preeclampsia increased significantly. NK cell in maternal peripheral blood and deciduas probably play a role in early onset preeclampsia.

Key words: early onset preeclampsia, Natural killer cell, deciduas

中图分类号:

赵秀伶, 马红梅, 马唯, 单志娟, 洛丽. NK细胞与早发型重度子痫前期疾病进展的相关性研究[J]. 首都医科大学学报, 2016, 37(4): 538-542.

Zhao Xiuling, Ma Hongmei, Ma Wei, Shan Zhijuan, Luo Li. Study about the correlation of NK cell with early onset preeclampsia[J]. Journal of Capital Medical University, 2016, 37(4): 538-542.

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NK细胞与早发型重度子痫前期疾病进展的相关性研究

Study about the correlation of NK cell with early onset preeclampsia

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