比较高碘酸-雪夫与双花扁豆凝集素反应标记的小鼠蜕膜自然杀伤细胞

doi:10.3969/j.issn.1006-7795.2013.01.022

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (1): 115-119.doi: 10.3969/j.issn.1006-7795.2013.01.022

比较高碘酸-雪夫与双花扁豆凝集素反应标记的小鼠蜕膜自然杀伤细胞

路欣¹, 许晴², 翁静³

1. 首都医科大学基础医学教学示范中心形态学实验室, 北京 100069;
2. 首都医科大学医学实验与测试中心, 北京 100069;
3. 首都医科大学基础医学院组织学与胚胎学教研室, 北京 100069

收稿日期:2012-07-04 出版日期:2013-02-21 发布日期:2013-02-25
通讯作者: 翁静 E-mail:wengjing@ccmu.edu.cn
基金资助:
北京市教育委员会科技发展计划资助项目(KM200910025005)。

Comparison of murine decidual natural killer cells shown by Periodic Acid Schiff's and Dolichos Bifows Agglutinin reactions

LU Xin¹, XU Qing², WENG Jing³

1. Morphological Laboratory, Center for Basic Medical Experimental Teaching, School of Basic Medical Science, Capital Medical University, Beijing 100069, China;
2. Medical Experiments and Testing Center, Capital Medical University, Beijing 100069, China;
3. Department of Histology and Embryology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China

Received:2012-07-04 Online:2013-02-21 Published:2013-02-25
Supported by:
This study was supported by Scientific Research Key Program of Beijing Municipal Commission of Education (KM200910025005).

摘要/Abstract

摘要：

目的对比高碘酸-雪夫(Periodic acid-Schiff,PAS)与双花扁豆凝集素(Dolichos Bifows Agglutinin,DBA)组织化学法所显示的小鼠蜕膜自然杀伤 (decidual natural killer,dNK) 细胞的不同。方法取妊娠第3~19天小鼠子宫,制备成连续的切片,分别经PAS、DBA组织化学法、DBA加PAS双重反应显示dNK细胞。结果典型的 dNK细胞含有PAS阳性颗粒,其胞膜、核膜和颗粒膜与DBA特异性结合而呈阳性反应;妊娠早期(妊娠第3~6天)不含颗粒的幼稚dNK细胞也可以被DBA标记。DBA加PAS双重反应时,含颗粒的dNK细胞大部分可以被同时标记。结论 DBA是dNK细胞的一种新的标志物,在妊娠早期可用以显示 PAS阳性颗粒尚未出现的幼稚dNK细胞。

关键词: 蜕膜自然杀伤细胞, PAS反应, 双花扁豆凝集素, 小鼠

Abstract:

To identify the difference of mouse decidual natural killer (dNK) cells reacting to Periodic Acid Schiff's (PAS) and Dolichos Biflorus agglutinin (DBA). Methods The dNK cells of mice uteri Gd 3 to Gd 19 (using the copulation plug as Gd1) were shown on continuous paraffin sections by PAS, DBA, PAS/DBA double reactions protocol. Results Typical dNK cells contained numerous PAS positive reactive cytoplasmic granules. The cytoplasmic membrane, nuclear membrane and granule membrane of dNK were positive reaction to DBA due to their specific binding. The immature dNK cells in the uterus of early pregnancy (on Gd 3 to Gd 6) were negative to PAS because of their absence of granules. Majority of dNK cells could be labeled at the same time with DBA plus PAS double staining. Conclusion DBA can be used as a new marker to show dNK due to its specific binding to membranes. DBA has advantage especially in early pregnancy (such as on particles Gd 3) because PAS positive granules have not yet appeared in immature dNK cells.

Key words: decidual natural killer cells, Periodic Acid-Schiff reaction, Dolichos Bifows Agglutinin, mouse

中图分类号:

R711

路欣, 许晴, 翁静. 比较高碘酸-雪夫与双花扁豆凝集素反应标记的小鼠蜕膜自然杀伤细胞[J]. 首都医科大学学报, 2013, 34(1): 115-119.

LU Xin, XU Qing, WENG Jing. Comparison of murine decidual natural killer cells shown by Periodic Acid Schiff's and Dolichos Bifows Agglutinin reactions[J]. Journal of Capital Medical University, 2013, 34(1): 115-119.

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比较高碘酸-雪夫与双花扁豆凝集素反应标记的小鼠蜕膜自然杀伤细胞

Comparison of murine decidual natural killer cells shown by Periodic Acid Schiff's and Dolichos Bifows Agglutinin reactions

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