原发性卵巢功能不全小鼠卵巢血流与性激素浓度相关性研究

doi:10.3969/j.issn.1006-7795.2017.06.023

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (6): 897-902.doi: 10.3969/j.issn.1006-7795.2017.06.023

原发性卵巢功能不全小鼠卵巢血流与性激素浓度相关性研究

冯逊逊¹, 董晓英², 郑秀蕊¹

1. 首都医科大学基础医学院, 北京 100069;
2. 首都医科大学中医药学院, 北京 100069

收稿日期:2017-06-26 出版日期:2017-11-21 发布日期:2017-12-16
通讯作者: 董晓英 E-mail:softsongs@126.com
基金资助:
北京市自然科学基金面上项目（7162026），北京中医药薪火传承"3+3"工程项目（2012-SZ-C-41），首都中医药科技专项（JJ2013-01），首都医科大学中医药专项课题（15ZY05）。

Correlative studies between haemodynamics and sex hormone concentrations in mice with primary ovarian insufficiency

Feng Xunxun¹, Dong Xiaoying², Zheng Xiurui¹

1. School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
2. Department of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China

Received:2017-06-26 Online:2017-11-21 Published:2017-12-16
Supported by:
This study was supported by Natural Science Foundation of Beijing(7162026), Eternal Flame "3+3" Project of Beijing Traditional Chinese Medicine(2012-SZ-C-41),Capital Traditional Chinese Medicine Project(JJ2013-01),Capital Medical University Traditional Chinese Medicine Project(15ZY05).

摘要/Abstract

摘要： 目的研究原发性卵巢功能不全（primary ovarian insufficiency，POI）小鼠卵巢的血流动力学与性激素浓度的相关性。方法以小鼠透明带3作为抗原，皮下多点注射，免疫BALB/C雌性小鼠建立原发性卵巢功能不全模型，利用超声动态观察卵巢的形态，同时利用超声彩色多普勒成像观察卵巢的血流变化，最后取小鼠血清进行性激素浓度检测。结果超声下可观察到模型组小鼠卵巢体积减小，卵泡数目减少，血流分布较弱。与空白组小鼠相比较，模型组小鼠阻力指数（resistive index，RI）较高，搏动指数（pulsatility index，PI，PI=S-D/Vmean）较低，脉压差（pulse pressure，PP）呈升高趋势；与空白组小鼠相比较，模型组小鼠的促卵泡生长激素（follicle-stimulating hormone，FSH）、抗苗勒管激素（anti-Müllerian hormone，AMH）均差异有统计学意义（P<0.05）。进一步通过模型组小鼠血流动力学与激素浓度相关性检验，发现模型组小鼠PP与FSH有一定（P<0.05）。结论 POI小鼠血流动力学PP与血清学FSH浓度有显著相关性，本研究为动态观察POI治疗过程提供参考。

关键词: 原发性卵巢功能不全, 超声血流, 动态, 性激素, 相关性

Abstract: Objective To study the correlation between haemodynamics and sex hormone concentrations in mice with premature ovarian insufficiency (POI).Methods BALB/c female mice model of POI was established by multiple sites subcutaneous injection of zona pellucida 3 of mice,and then ovaries were observed by applying ultrasound meanwhile hemodynamics changes were recorded by ultrasound color doppler. Finally, mice serum was taken to be detected.Results Under the images of ultrasound, ovaries of mice in model group experience the volume decrease, follicle decrease and low blood flow. Compared to the blank group, mice of model group have lower pulsatility index (PI), higher resistance index (RI) and an ascending trend of pulse pressure (PP). Moreover, the follicle-stimulating hormone (FSH) and anti-Müllerian hormone (AMH) in mice of model group had a significant difference (P<0.05) compared to the blank group. Then, through the correlation test between haemodynamics and sex hormone concentrations, there was a significant difference in PP and FSH (P<0.05).Conclusion The PP in haemodynamics and FSH in sex hormone concentrations have a significant difference and it can provide the base for dynamic observation in course of treatment.

Key words: POI, ultrasound blood flow, dynamic, sex hormone, correlation

中图分类号:

R711.75

冯逊逊, 董晓英, 郑秀蕊. 原发性卵巢功能不全小鼠卵巢血流与性激素浓度相关性研究[J]. 首都医科大学学报, 2017, 38(6): 897-902.

Feng Xunxun, Dong Xiaoying, Zheng Xiurui. Correlative studies between haemodynamics and sex hormone concentrations in mice with primary ovarian insufficiency[J]. Journal of Capital Medical University, 2017, 38(6): 897-902.

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原发性卵巢功能不全小鼠卵巢血流与性激素浓度相关性研究

Correlative studies between haemodynamics and sex hormone concentrations in mice with primary ovarian insufficiency

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