人胎盘间充质干细胞治疗小鼠流产的效果研究

doi:10.3969/j.issn.1006-7795.2019.03.020

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (3): 434-438.doi: 10.3969/j.issn.1006-7795.2019.03.020

人胎盘间充质干细胞治疗小鼠流产的效果研究

周平¹, 刘程², 王秋石², 朱志清², 曹广明², 刘崇东², 张震宇²

1. 首都医科大学附属北京朝阳医院手术室, 北京 100020;
2. 首都医科大学附属北京朝阳医院妇产科, 北京 100020

收稿日期:2018-07-02 出版日期:2019-05-21 发布日期:2019-06-13
通讯作者: 张震宇 E-mail:zhangzhenyu_gem@163.com
基金资助:
吴阶平医学基金会临床科研专项资助基金（320.6755.15019）。

Therapeutic effect of placenta-derived mesenchymal stem cells on recurrent spontaneous abortion

Zhou Ping¹, Liu Cheng², Wang Qiushi², Zhu Zhiqing², Cao Guangming², Liu Chongdong², Zhang Zhenyu²

1. Operating Room, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China;
2. Department of Obstetric and Gynecologic, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China

Received:2018-07-02 Online:2019-05-21 Published:2019-06-13
Supported by:
This study was supported bySpecial Fund for Clinical Research by Wu Jieping Medical Foundation (320.6755.15019).

摘要/Abstract

摘要： 目的探索人胎盘间充质干细胞对复发性流产小鼠的治疗作用。方法建立CBA/J雌性×DBA/2J雄性复发性流产小鼠模型，分为注射人胎盘间充质干细胞（placenta-derived mesenchymal stem cells，PMSC）的实验组和注射磷酸盐缓冲液（PBS）的对照组，每组6只小鼠。比较两组的胚胎吸收率、胎鼠和胎盘质量。采用免疫组织化学的方法检测胎盘Laminin和Cytokeratin 8蛋白的表达情况。结果两组小鼠无过敏、排异或死亡等异常反应。实验组小鼠的胚胎吸收率为8.33%（5/60），对照组小鼠的胚胎吸收率为28.33%（17/60），两组比较，差异有统计学意义（χ²=8.01，P=0.005）。实验组胎鼠质量为（1 127.59±87.92） g，胎盘质量为（674.35±66.29） g；对照组胎鼠质量为（981.57±118.25） g，胎盘质量为（572.24±89.25） g，两组比较，差异有统计学意义（P=0.036，P=0.047）。实验组Laminin蛋白表达高于实验组，胎儿血管分支明显增多，胎儿血管总面积以及母体血窦总面积均显著升高。实验组侵润到蜕膜组织的Cytokeratin 8（CK8）阳性滋养层细胞的数量明显高于对照组，滋养层细胞向蜕膜迁移的距离显著远于对照组。结论 PMSC对流产小鼠具有安全性，且大大降低了小鼠的胚胎吸收率，为复发性流产的治疗提供新思路。

关键词: 人胎盘间充质干细胞, 复发性流产, 治疗, 动物实验

Abstract: Objective To investigate the therapeutic effect of placenta-derived mesenchymal stem cells (PMSC) on recurrent spontaneous abortion (RSA).Methods Female CBA/J mice were mated with male DBA/2 mice in 2:1 to construct RSA models (n=12). The RSA models were randomly divided into the experimental group and control group, six mice in each group. Mice in the experimental group were injected with PMSC and mice in the control groups were injected with PBS. The mice were killed on day 13.5 of pregnancy,and we calculated the abortion rate,and the weight of embryonic rat and placenta. The placental tissues were also detected to identify the Laminin and Cytokeratin 8 by immunohistochemistry.Results The embryo resorption rates in the experimental group were under the control group (P<0.05),and the weight of embryonic rat and placenta in the experimental group were higher than that of the control group (P<0.05).Conclusion The embryo resorption rate in RSA mice is decreased after injected with PMCS,providing new ideas for the treatment of recurrent spontaneous abortion.

Key words: placenta-derived mesenchymal stem cells, recurrent spontaneous abortion, therapy, animal research

中图分类号:

R714.21

周平, 刘程, 王秋石, 朱志清, 曹广明, 刘崇东, 张震宇. 人胎盘间充质干细胞治疗小鼠流产的效果研究[J]. 首都医科大学学报, 2019, 40(3): 434-438.

Zhou Ping, Liu Cheng, Wang Qiushi, Zhu Zhiqing, Cao Guangming, Liu Chongdong, Zhang Zhenyu. Therapeutic effect of placenta-derived mesenchymal stem cells on recurrent spontaneous abortion[J]. Journal of Capital Medical University, 2019, 40(3): 434-438.

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人胎盘间充质干细胞治疗小鼠流产的效果研究

Therapeutic effect of placenta-derived mesenchymal stem cells on recurrent spontaneous abortion

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