克氏综合征胎儿脐带间充质干细胞模型的建立

doi:10.3969/j.issn.1006-7795.2019.06.012

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (6): 868-874.doi: 10.3969/j.issn.1006-7795.2019.06.012

克氏综合征胎儿脐带间充质干细胞模型的建立

罗建安, 杨树法, 于秀义, 刘欣, 陈振文

首都医科大学基础医学院医学遗传学与发育生物学学系, 北京 100069

收稿日期:2019-04-23 出版日期:2019-11-21 发布日期:2019-12-18
通讯作者: 陈振文 E-mail:czwen@ccmu.edu.cn
基金资助:
国家自然科学基金（31772545），北京市教育委员会科技计划（KM201910025027）。

Establishment a mesenchymal stem cell line model derived from Klinefelter syndrome fetal umbilical cord

Luo Jianan, Yang Shufa, Yu Xiuyi, Liu Xin, Chen Zhenwen

Department of Medical Genetics and Developmental Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2019-04-23 Online:2019-11-21 Published:2019-12-18
Supported by:
This study was supported by National Natural Science Foundation of China (31772545),Science and Technology Plan of Beijing Education Commission (KM201910025027).

摘要/Abstract

摘要： 目的建立克氏综合征胎儿脐带间充质干细胞细胞系（Klinefelter’s syndrome umbilical cord mesenchymal stem cells，KUMSCs），使之用于克氏综合征的研究。方法从临床医院获取患有Klinefelter综合征的流产胎儿脐带，采用体外脐带间充质干细胞分离培养得到间充质干细胞。经质量检测、流式细胞术、核型分析、细胞诱导分化实验等技术进行鉴定。结果获得的KUMSCs经细菌、真菌、病毒、螺旋体、支原体、内毒素等检测均符合质量控制标准；流式细胞技术检测显示KUMSCs对CD73、CD90、CD29、CD105和CD44呈阳性反应，对CD34、CD45和人白细胞抗原-DR （human leukocyte antigen-DR，HLA-DR））呈阴性反应。细胞诱导分化实验结果表明KUMSCs可定向诱导分化为脂肪细胞、成骨细胞。结论成功建立了Klinefelter综合征胎儿脐带间充质干细胞模型，可应用于Klinefelter综合征发病机制、药物开发等实验研究。

关键词: Klinefelter综合征, 脐带间充质干细胞, 成骨分化, 成脂分化

Abstract: Objective We succeeded in establishing a mesenchymal stem cell line (Klinefelter's syndrome umbilical cord mesenchymal stem cells, KUMSCs) from Klinefelter syndrome (KS) fetal umbilical cord for the klinefelter's syndrome disease research. Methods Umbilical cords of aborted fetuses with Klinefelter syndrome were obtained from the clinical hospital. KUMSCs were isolated and expanded in vitro, and the analytical techniques included:quality detection, flow cytometry, karyotype analysis and cell differentiation experiments. Results The content of pathogens (bacteria, fungi, viruses, spirochetes, mycoplasma) and endotoxin of mesenchymal stem cells were all in line with the quality control standards, KUMSCs cells were positive for CD73, CD90, CD29, CD105 and CD44, negative for CD34, CD45 and human leukocyte antigen-DR(HLA-DR). Cell differentiation assay results indicate that KUMSCs can be induced to differentiate into adipocytes, osteoblasts. Conclusion These results indicate that a model of umbilical cord mesenchymal stem cells derived from KS has been successfully established, which can be applied to the experimental study of the pathogenesis and drug development of Klinefelter syndrome.

Key words: Klinefelter syndrome, umbilical cord mesenchymal stem cells, osteogenic differentiation, adipogenic differentiation

中图分类号:

R394

罗建安, 杨树法, 于秀义, 刘欣, 陈振文. 克氏综合征胎儿脐带间充质干细胞模型的建立[J]. 首都医科大学学报, 2019, 40(6): 868-874.

Luo Jianan, Yang Shufa, Yu Xiuyi, Liu Xin, Chen Zhenwen. Establishment a mesenchymal stem cell line model derived from Klinefelter syndrome fetal umbilical cord[J]. Journal of Capital Medical University, 2019, 40(6): 868-874.

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克氏综合征胎儿脐带间充质干细胞模型的建立

Establishment a mesenchymal stem cell line model derived from Klinefelter syndrome fetal umbilical cord

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