MALAT1在慢性鼻窦炎伴鼻息肉中的表达及对糖皮质激素治疗的反应性

doi:10.3969/j.issn.1006-7795.2020.02.007

首都医科大学学报 ›› 2020, Vol. 41 ›› Issue (2): 183-188.doi: 10.3969/j.issn.1006-7795.2020.02.007

• 慢性鼻窦炎伴鼻息肉的基础研究 • 上一篇下一篇

MALAT1在慢性鼻窦炎伴鼻息肉中的表达及对糖皮质激素治疗的反应性

王明, 卜祥婷, 栾格, 王阳, 王成硕, 张罗

首都医科大学附属北京同仁医院耳鼻咽喉头颈外科北京市耳鼻咽喉科研究所鼻病研究北京市重点实验室, 北京 100730

收稿日期:2020-01-14 出版日期:2020-04-21 发布日期:2020-04-16
通讯作者: 张罗 E-mail:dr.luozhang@139.com
基金资助:
国家重点研发计划（2016YFC0905200），国家自然科学基金（81800882、81630023、81870698），教育部长江学者及创新团队发展计划（IRT13082），北京市医院管理局使命人才计划（SML20150203）。

Expression and glucocorticoid regulation of MALAT1 in chronic rhinosinusitis with nasal polyps

Wang Ming, Bu Xiangting, Luan Ge, Wang Yang, Wang Chengshuo, Zhang Luo

Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University;Beijing Institute of Otorhinolaryngology;Beijing Key Laboratory of Nasal Diseases, Beijing 100730, China

Received:2020-01-14 Online:2020-04-21 Published:2020-04-16
Supported by:
This study was supported by National Key R&D Program of China （2016YFC0905200）， National Natural Science Foundation of China （81800882， 81630023， 81870698）， Program for Changjiang Scholars and Innovative Research Team （IRT13082）， the Municipal Administration of Hospitals’Mission Plan （SML20150203）.

摘要/Abstract

摘要： 目的研究长链非编码RNA肺腺癌转移相关转录本1（metastasis-associated lung adenocarcinoma transcript 1，MALAT1）在不同亚型慢性鼻窦炎伴鼻息肉（chronic rhinosinusitis with nasal polyps，CRSwNP）中的表达，及其对糖皮质激素治疗的反应性。方法利用real-time PCR检测MALAT1在嗜酸性粒细胞型和非嗜酸性粒细胞型CRSwNP鼻息肉组织及健康对照鼻黏膜组织中的表达水平，分析MALAT1表达与Th1、Th2、Th17型细胞因子表达的相关性，并比较同一患者经糖皮质激素治疗前后MALAT1的表达变化。结果 MALAT1在两种亚型CRSwNP鼻黏膜中的表达均显著低于健康对照组，并且在非嗜酸性粒细胞型CRSwNP组低于嗜酸性粒细胞型CRSwNP组；干扰素-γ（interferon gamma，IFNG）的表达在嗜酸性粒细胞型CRSwNP和非嗜酸性粒细胞型CRSwNP均显著高于健康对照组；MALAT1的表达与IFNG的表达呈显著负相关（r=-0.595，P=0.001）。糖皮质激素治疗能显著上调不同亚型CRSwNP中MALAT1的表达（P=0.015）。结论 MALAT1在不同亚型CRSwNP中显著低表达，且与IFNG的表达呈负相关，糖皮质激素能上调MALAT1的表达。

关键词: 慢性鼻窦炎伴鼻息肉, 肺腺癌转移相关转录本1, 干扰素-γ, 糖皮质激素

Abstract: Objective To explore the expression of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in different subtypes of chronic rhinosinusitis with nasal polyps (CRSwNP) and its response to glucocorticoid therapy. Methods Real-time PCR was used to detect the expression of MALAT1 in nasal tissues from eosinophilic CRSwNP, non-eosinophilic CRSwNP and healthy control subjects. The correlation between the expression of MALAT1 and Th1/Th2/Th17 cytokines was analyzed. Moreover, the effect of glucocorticoid treatment on MALAT1 expression was measured. Results MALAT1 was significantly down-regulated in both eosinophilic CRSwNP and non-eosinophilic CRSwNP compared to the healthy control. Moreover, the expression of MALAT1 was significantly lower in non-eosinophilic CRSwNP than that of eosinophilic CRSwNP. In contrast, interferon gamma (IFNG) was significantly up-regulated in both eosinophilic CRSwNP and non-eosinophilic CRSwNP compared to the healthy control. The Spearman correlation test showed that the expression of MALAT1 was negatively correlated with the expression of IFNG (r=-0.595,P=0.001). In addition, MALAT1 was significantly up-regulated by glucocorticoid treatment both in patients with eosinophilic CRSwNP and non-eosinophilic CRSwNP (P=0.015, respectively). Conclusion MALAT1 is significantly down-regulated in both eosinophilic CRSwNP and non-eosinophilic CRSwNP compared to the healthy control, and it negatively correlated with the expression of IFNG. Glucocorticoid treatment significantly up-regulate the expression of MALAT1 in CRSwNP.

Key words: chronic rhinosinusitis with nasal polyps, metastasis-associated lung adenocarcinoma transcript 1(MALAT1), interferon gamma, glucocorticoid

中图分类号:

R765

王明, 卜祥婷, 栾格, 王阳, 王成硕, 张罗. MALAT1在慢性鼻窦炎伴鼻息肉中的表达及对糖皮质激素治疗的反应性[J]. 首都医科大学学报, 2020, 41(2): 183-188.

Wang Ming, Bu Xiangting, Luan Ge, Wang Yang, Wang Chengshuo, Zhang Luo. Expression and glucocorticoid regulation of MALAT1 in chronic rhinosinusitis with nasal polyps[J]. Journal of Capital Medical University, 2020, 41(2): 183-188.

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MALAT1在慢性鼻窦炎伴鼻息肉中的表达及对糖皮质激素治疗的反应性

Expression and glucocorticoid regulation of MALAT1 in chronic rhinosinusitis with nasal polyps

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