超声引导股动脉穿刺置管在血管内介入方法制备恒河猴急性脑梗死模型中的应用

doi:10.3969/j.issn.1006-7795.2021.02.013

首都医科大学学报 ›› 2021, Vol. 42 ›› Issue (2): 239-242.doi: 10.3969/j.issn.1006-7795.2021.02.013

• 脑缺血损伤的基础研究 • 上一篇下一篇

超声引导股动脉穿刺置管在血管内介入方法制备恒河猴急性脑梗死模型中的应用

马艳辉¹, 吴洁¹, 贾宾¹, 姚东旭¹, 徐娜¹, 王天龙¹, 陈健², 吴迪^2,*

1. 首都医科大学宣武医院麻醉手术科,北京 100053;
2. 首都医科大学宣武医院中美神经科学研究所,北京 100053

收稿日期:2021-01-14 发布日期:2021-04-26
基金资助:
国家自然科学基金(81871022,82071466)

Application of ultrasound-guided femoral artery puncture and catheterization on endovascular ischemic stroke models of adult rhesus monkeys

Ma Yanhui¹, Wu Jie¹, Jia Bin¹, Yao Dongxu¹, Xu Na¹, Wang Tianlong¹, Chen Jian², Wu Di^{2, *}

1. Department of Anesthesiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. China-America Institute of Neuroscience, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Received:2021-01-14 Published:2021-04-26
Contact: ^*E-mail:di.wu@xwhosp.org
Supported by:
This study was supported by National Natural Science Foundation of China (81871022,82071466).

摘要/Abstract

摘要： 目的探讨超声引导股动脉穿刺置管在血管内介入方法制备恒河猴急性脑梗死模型中的应用效果。方法选择拟行大脑中动脉M2段栓塞建模的健康成年恒河猴16只,雄性,采用数字表法随机分为2组(n=8):对照组(C组)、超声引导组(U组)。所有恒河猴采用0.1 mL/kg氯胺酮及速眠新的混合液肌内注射进行基础麻醉,待动物入睡后行气管内插管,接呼吸机行机械通气,麻醉维持采用持续输注丙泊酚(5~8 mg·kg^-1·h^-1)。C组采用传统指尖触摸法进行股动脉穿刺置管,U组采用超声引导平面内-平面外联合法进行股动脉穿刺置管。比较两组1次、2次穿刺置管成功率、总成功率、穿刺置管次数、穿刺置管花费时间及并发症发生率。结果 C组与U组1次穿刺置管成功率分别为25.0%和62.5%,2次穿刺置管成功率分别为50.0%和87.5%、总成功率分别为75.0%和100%。C组与U组穿刺置管次数分别为(3.0±1.6)次和(1.5±0.8)次,穿刺置管花费时间分别为(15.8±6.1) min和(5.4±2.9)min。与C组比较,U组穿刺置管次数少,穿刺置管花费时间较短,两组差异有统计学意义(P<0.05);与C组比较,U组1次、2次穿刺置管成功率及总成功率均较高,但差异无统计学意义(P>0.05)。两组股动脉穿刺置管并发症发生率比较差异无统计学意义(P>0.05)。结论在血管内介入方法制备恒河猴急性脑梗死模型中,采用平面内-平面外联合技术进行超声引导股动脉穿刺,可提高首次穿刺置管成功率及总成功率,减少穿刺置管次数,缩短穿刺置管花费时间。

关键词: 恒河猴, 大脑中动脉栓塞, 股动脉穿刺置管, 超声引导

Abstract: Objective Investigate the effect of ultrasound-guided femoral artery puncture and catheterization in the development of ischemic stroke models of adult rhesus monkeys through endovascular methods.Methods Sixteen male rhesus monkeys were randomized into 2 groups: control group (Group C) and ultrasound-guided group (Group U). Su-mian-xin injection combined with 0.1 mL/kg ketamine was given before endotracheal intubation. After intubation, the animals received mechanical ventilation. Anesthesia was maintained with propofol infusion (5-8 mg·kg^-1·h^-1) in both groups. Classic palpation method was used for femoral artery puncture and catheterization in Group C. Ultrasound-guidance combined in-plane and out-of-plane was used for femoral artery puncture and catheterization in Group U. The first attempt success rate, second attempt success rate, total success rate, numbers of attempts, period of puncture and catheterization time and incidence of complications were compared between two groups. Results The frequency of attempts in Group U was less than that in Group C (P<0.05). Compared with Group C, the mean time to success was shorter in Group U (P<0.05). The success rate of the first and second attempt and total success rates were high in Group U, with no statistical differences (P>0.05). There was no difference in the incidence of complications between the two groups (P>0.05). Conclusion Ultrasound-guidance combined in-plane and out-of-plane used for femoral artery puncture and catheterization improved the initial attempt success rate and total success rate, reduce the frequency of attempts and the mean time on endovascular ischemic stroke models of adult rhesus monkeys.

Key words: rhesus monkeys, middle cerebral artery occlusion, femoral artery puncture and catheterization, ultrasound-guided

中图分类号:

R743

马艳辉, 吴洁, 贾宾, 姚东旭, 徐娜, 王天龙, 陈健, 吴迪. 超声引导股动脉穿刺置管在血管内介入方法制备恒河猴急性脑梗死模型中的应用[J]. 首都医科大学学报, 2021, 42(2): 239-242.

Ma Yanhui, Wu Jie, Jia Bin, Yao Dongxu, Xu Na, Wang Tianlong, Chen Jian, Wu Di. Application of ultrasound-guided femoral artery puncture and catheterization on endovascular ischemic stroke models of adult rhesus monkeys[J]. Journal of Capital Medical University, 2021, 42(2): 239-242.

参考文献

[1] Wu D, Chen J, Hussain M, et al.Selective intra-arterial brain cooling improves long-term outcomes in a non-human primate model of embolic stroke: Efficacy depending on reperfusion status[J]. J Cereb Blood Flow Metab, 2020,40(7):1415-1426.
[2] 马艳辉, 吴迪, 田肇隆, 等. 两种麻醉方法用于恒河猴急性脑梗死模型麻醉效果的比较[J]. 首都医科大学学报, 2018, 39(4):552-556.
[3] 朱义, 杜真, 汪丽娜, 等. 超声引导在婴儿桡动脉穿刺置管中的应用[J]. 临床小儿外科杂志, 2019, 18(8):699-702.
[4] 陈雪,张文超,王佳, 等. 超声引导技术在老年髋部骨折患者桡动脉穿刺置管中的应用[J]. 医学综述, 2018, 24(23):4600-4604.
[5] Fisher M, Feuerstein G, Howells D W, et al.Update of the stroke therapy academic industry roundtable preclinical recommendations[J]. Stroke,2009,40(6): 2244-2250.
[6] 吴迪, 何小夺, 陈健, 等. 血管内介入方法制备非人灵长类动物局部脑缺血模型的研究进展[J]. 中国比较医学杂志, 2018, 28(5):16-20.
[7] Ishii S, Shime N, Shibasaki M, et al.Ultrasound-guided radial artery catheterization in infants and small children[J]. Pediatr Crit Care Med, 2013,14(5):471-473.
[8] Gao Y B,Yan J H,Gao F Q,et al.Effects of ultrasound-guided radial artery catheterization: an updated meta-analysis[J]. Am J Emerg Med, 2015, 33(1):50-55.
[9] 吕炎基, 余璞琳, 刘淼, 等. 超声引导下血管穿刺长短轴的选择:Meta分析[J]. 医学研究杂志, 2018, 47(10):45-48.
[10] 权哲峰, 池萍, 李昕, 等. 超声引导下桡动脉穿刺中短轴与长轴方法的应用[J]. 临床麻醉学杂志, 2015, 31(5):467-468.
[11] 陈广俊, 许楠, 唐帅, 等. 平面内-平面外技术相结合的超声引导下桡动脉穿刺置管策略[J]. 北京医学, 2013, 35(6):453-457.
[12] 欧阳惠碧, 蔡建历, 李志鹏, 等. 超声引导足背动脉穿刺置管的临床观察[J]. 临床超声医学杂志, 2018, 20(12):864, 封3.
[13] 马进. 超声引导下桡动脉穿刺置管在老年患者中的应用效果[J]. 临床医学研究与实践, 2020, 5(7):83-84.
[14] Gutzeit A, Schoch E, Sautter T, et al.Antegrade access to the superficial femoral artery with ultrasound guidance: feasibility and safety[J]. J Vasc Interv Radiol, 2010, 21(10):1495-1500.

超声引导股动脉穿刺置管在血管内介入方法制备恒河猴急性脑梗死模型中的应用

Application of ultrasound-guided femoral artery puncture and catheterization on endovascular ischemic stroke models of adult rhesus monkeys

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

[1]	赖光辉, 张达谦, 倪家骧. 影像引导下蝶腭神经节联合选择性颈神经阻滞治疗蝶腭神经痛的回顾性分析[J]. 首都医科大学学报, 2020, 41(2): 272-276.
[2]	马艳辉, 吴迪, 田肇隆, 贾宾, 徐娜, 陈健, 王天龙. 两种麻醉方法用于恒河猴急性脑梗死模型麻醉效果的比较[J]. 首都医科大学学报, 2018, 39(4): 552-556.
[3]	谢海, 李艳. 超声引导下不同入路腹横肌平面阻滞对剖宫产手术后镇痛的影响[J]. 首都医科大学学报, 2015, 36(5): 791-793.
[4]	岳剑宁, 武百山, 王琦, 郭玉娜, 刘京杰, 何明伟, 李玄英, 倪家骧. 超声引导下星状神经节阻滞有效性及安全性评价[J]. 首都医科大学学报, 2014, 35(1): 32-34.
[5]	兰飞, 王天龙. 0.5%罗哌卡因用于超声引导经臀下入路坐骨神经阻滞的半数有效剂量[J]. 首都医科大学学报, 2013, 34(5): 666-668.
[6]	谷晔炳;李坤成;姚新宇;张进禄;刘玉军;鲁强;徐群渊. 甲基-苯基-四氢吡啶建立恒河猴PD模型的MRI表现[J]. 首都医科大学学报, 2000, 21(3): 187-189.
[7]	刘玉军;张国风;鲁强. 猴运动皮质损伤模型的建立[J]. 首都医科大学学报, 1992, 13(4): 264-268.