不同时间低压低氧刺激对大鼠肺动脉压力及肺组织的影响

doi:10.3969/j.issn.1006-7795.2021.04.014

首都医科大学学报 ›› 2021, Vol. 42 ›› Issue (4): 596-600.doi: 10.3969/j.issn.1006-7795.2021.04.014

不同时间低压低氧刺激对大鼠肺动脉压力及肺组织的影响

邓会¹, 王勇², 陈小兰¹, 秦崇¹, 潘磊^1*

1.首都医科大学附属北京世纪坛医院呼吸与危重症医学科低压低氧实验室,北京 100038;
2.北京急救中心, 北京 100031

收稿日期:2020-08-27 出版日期:2021-08-21 发布日期:2021-07-29
基金资助:
高原铁路工作人员轮休制度的基础研究 (2020-A36)。

Effects of hypobaric hypoxia stimulation at different times on pulmonary arterial pressure and lung tissue in rats

Deng Hui¹, Wang Yong², Chen Xiaolan¹, Qin Chong¹, Pan Lei^1*

1. Department of Respiratory and Critical Care Medicine, Hypobaric Hypoxia Laboratory, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China;
2. Beijing Emergency Center, Beijing 100031, China

Received:2020-08-27 Online:2021-08-21 Published:2021-07-29
Contact: * E-mail:leipan2010@163.com
Supported by:
Basic Research on the Rotation System of Plateau Railway Staff (2020-A36).

摘要/Abstract

摘要： 目的观察不同低压低氧刺激时间对大鼠平均肺动脉压力、肺组织及酪氨酸激酶中血管内皮生长因子(vascular endothelial growth factor,VEGF)、血小板源性生长因子(platelet derived growth factor,PDGF)、成纤维细胞生长因子(fibroblast growth factor,FGF)表达水平的影响。方法将32只雄性SD大鼠采用抽签法随机分为4组,A组正常海拔环境下饲养21 d;B组持续低压低氧环境(低压低氧舱模拟海拔5 000米高度)21 d,C组每天6 h低压低氧环境持续21 d,D组间断24 h(隔天)低压低氧环境21 d,光照、饮食、饮水等因素相同。利用BL-420S生物信号采集系统,通过右心导管法测量每组大鼠肺动脉压力;HE染色观察每组大鼠肺组织;ELISA方法测定每组大鼠血清中VEGF、PDGF、FGF表达量。结果 ①B、C、D组大鼠平均肺动脉压力较A组升高(P＜0.05),其中B组升高最明显,平均肺动脉压力为(27.4±1.2) mmHg(1 mmHg=0.133 kPa),其次为D组,平均肺动脉压力为(22.1±1.1) mmHg。②B、C、D组大鼠肺血管周围均可见淋巴细胞浸润,B、D组大鼠肺组织可见炎症细胞及纤维组织引起的局部实变,亦可见局部肺泡过度充气,呈肺气肿表现。③B、C、D组VEGF、PDGF、FGF表达量较A组明显升高(P＜0.05)。结论间断低压低氧刺激可以诱发大鼠肺动脉压力升高,但是间断低压低氧6 h、间断低压低氧24 h持续21 d的大鼠平均肺动脉压力未达到25 mmHg;间断低压低氧导致肺内炎症细胞浸润增多、肺泡壁结构破坏,进而造成肺实变、肺气肿病变;VEGF、PDGF、FGF等酪氨酸激酶表达量明显升高。

关键词: 低压低氧, 肺动脉压力, 肺组织

Abstract: Objective To observe the effects of different hypobaric and hypoxic stimulation time on the average pulmonary artery pressure, the expression levels of the vascular endothelial growth factor (VEGF), the platelet derived growth factor(PDGF) and the fibroblast growth factor(FGF) in serum of rats. Methods Thirty two male SD rats were divided into four groups: group A was fed for 21 days at normal altitude, group B was kept in hypobaric hypoxia environment for 21 days (simulated altitude of 5 000 meters in hypobaric hypoxia chamber), group C was 6 hours a day in hypobaric hypoxia environment for 21 days, group D was interrupted for 24 hours (the next day)in hypobaric hypoxia environment for 21 days, and the factors of light, diet and water were the same. The pulmonary artery pressure of each group was measured by right cardiac catheterization, the lung tissue of each group was observed by HE staining, and the expression of VEGF, PDGF and FGF in serum of each group was measured by ELISA. Results ① the average pulmonary artery pressure in group B, C and D was higher than that in group A (P<0.05), the most significant increase was (27.4±1.2) mmHg (1 mmHg=0.133 kPa) in group B, followed by (22.1±1.1) mmHg in Group D. ② In group B, C and D, lymphocyte infiltration was found around the pulmonary vessels. In group B and D, inflammatory cells and fibrous tissue caused local consolidation were found in the lung tissue, and local alveolar hyperinflation was also found, presenting as emphysema. ③ The expression of VEGF, PDGF and FGF in group B, C and D were significantly higher than that in group A (P<0.05). Conclusion Intermittent hypobaric hypoxia stimulation can increase pulmonary arterial pressure in rats, but the average pulmonary artery pressure of rats with intermittent hypobaric hypoxia for 6 hours or 24 hours did not reach 25 mmHg during 21 days; Intermittent hypobaric hypoxia leads to the increase of inflammatory cell infiltration and the destruction of alveolar wall structure, which leads to pulmonary consolidation and emphysema; The expression of VEGF, PDGF, FGF was significantly increased.

Key words: hypobaric hypoxia, pulmonary artery pressure, lung tissue

中图分类号:

邓会, 王勇, 陈小兰, 秦崇, 潘磊. 不同时间低压低氧刺激对大鼠肺动脉压力及肺组织的影响[J]. 首都医科大学学报, 2021, 42(4): 596-600.

Deng Hui, Wang Yong, Chen Xiaolan, Qin Chong, Pan Lei. Effects of hypobaric hypoxia stimulation at different times on pulmonary arterial pressure and lung tissue in rats[J]. Journal of Capital Medical University, 2021, 42(4): 596-600.

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不同时间低压低氧刺激对大鼠肺动脉压力及肺组织的影响

Effects of hypobaric hypoxia stimulation at different times on pulmonary arterial pressure and lung tissue in rats

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