血浆血管紧张素Ⅱ高压液相层析—放射免疫结合技术测定及巯甲丙脯酸抑制其生成的研究

首都医科大学学报 ›› 1987, Vol. 8 ›› Issue (3): 204-208.

血浆血管紧张素Ⅱ高压液相层析—放射免疫结合技术测定及巯甲丙脯酸抑制其生成的研究

于仲元, 汪家瑞, 何士大, 徐宝钠, 张叔诚, 洪方裕, 宋若华, 黄丽君

首都医学院宣武医院心血管病研究室首都医学院宣武医院高血压实验室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1987-07-15 发布日期:1987-07-15

The Measurement of Plasma Angiotensin Ⅱ by HPLCRIA and the Observation on the Inhibition of the Plasma Angiotensin Ⅱ Generation by Captopril

Yu Zhongyuan

Cardiovasular Research Lab Xuanwu Hospital Capital Institute of Medicine

Received:1900-01-01 Revised:1900-01-01 Online:1987-07-15 Published:1987-07-15

摘要/Abstract

摘要： 血浆血管紧张素Ⅱ(PATⅡ)经Sep-pakC¹⁸柱和高压液相层析(HPLC)分离,放射免疫(RIA)测定。并通过8例原发性高血压病人进行了巯甲丙脯酸抑制PATⅡ生成的研究。结果显示,口服25mg巯甲丙脯酸后10分钟,PATⅡ水平即有显著下降(P<0.01),60分钟时达最低水平(P<0.02)。平均动脉压(MAP)亦同时达到最低水平(P<0.02)。下降率为34%。60分钟后,APTⅡ,MAP均逐渐回升。血浆肾素活性(PRA)在用药后30分钟内未见显著性变化。PRA水平反馈增高,但与MAP变化无同时达到峰值的相应关系。

关键词: 血管紧张素 Ⅱ, 巯甲丙脯酸, 高压液相层析, 放射免疫

Abstract: The plasma angiotensin II(ATII)has been separated by Sep-Pak C¹³and purified by HPLC then measured by RIA.25 mg of Captopril was administred orally to 8 patients with essential hypertension. The significant change of Plasma ATII started from 10 minutes after administration of Captopril.Plasma ATII and MAP both reached the lowest level at 60 minutes after Captopril,such response between plasma renin activity(PRA)and MAP was not observed.

Key words: AngiotensinⅡ, Captopril, HPLC, RIA

于仲元;汪家瑞;何士大;徐宝钠;张叔诚;洪方裕;宋若华;黄丽君. 血浆血管紧张素Ⅱ高压液相层析—放射免疫结合技术测定及巯甲丙脯酸抑制其生成的研究[J]. 首都医科大学学报, 1987, 8(3): 204-208.

Yu Zhongyuan. The Measurement of Plasma Angiotensin Ⅱ by HPLCRIA and the Observation on the Inhibition of the Plasma Angiotensin Ⅱ Generation by Captopril[J]. Journal of Capital Medical University, 1987, 8(3): 204-208.

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