首都医科大学学报 ›› 1989, Vol. 10 ›› Issue (3): 188-192.

• 论著 • 上一篇    下一篇

小鼠早期胚胎原始生殖细胞的组织化学研究

诸定寿, 刘英琴, 史小林, 路欣   

  1. 首都医学院组织胚胎学教研室
  • 收稿日期:1989-03-10 修回日期:1900-01-01 出版日期:1989-07-15 发布日期:1989-07-15

The Study of Histochemistry in Primordial Germ Cells of the Early Mouse Embryo

Zhu Dingshou, Liu Yingqin, Shi Xiaolin, Lu Xin   

  1. Department of Histology and Embryology, Capital Institute of Medicine
  • Received:1989-03-10 Revised:1900-01-01 Online:1989-07-15 Published:1989-07-15

摘要: 对妊娠12.5~16.5d小鼠胚胎的原始性腺做恒冷箱切片与胶原酶分离标本进行组织化学研究。结果证明,原始生殖细胞含有丰富的碱性磷酸酶(AKP)和亮氨酸氨基肽酶(LNAse),后者为作者第一次发现。酸性磷酸酶(ACP),非特异性酯酶(NSE),葡萄糖-6-磷酸酶(G-6-Pase),琥珀酸脱氢酶(SDH),3-磷酸腺苷酶(ATPase),糖原及脂类含量极少。用AKP染色可把原始生殖细胞分为大、中和小3类,后者与体细胞很难区别。

关键词: 原始生殖细胞, 小鼠, 组织化学

Abstract: The study defines the primordial Germ cells (PGCs) of the Early Mouse Embryo containing ALKaline Phosphatase (AKP). The other somal cells did not contain AKP. According to that, AKP is the marked enzyme of PGCs. The AKP is localized the in cytoplasm of PGCs, but the nucleus was negative. LNAse is strongly positive and it was found by the author. LNAse was more positive than AKP in the PGCs. I think that LNAse also may be the enzyme made by PGCs. There has been no report about the LNAse in PGCs. There are three kinds of PGCs. The big ceils are 30μm, the midile cells are 15-20μm and the small cells are 10μm in diameter. The big cells are different from the somal cells and the small cells are difficult to differentiate from the somal cells. We have studied ACP, NSE, ATPase, SDH, Lipin, Glycogen and G-6- Pase. They all were weakly positive or negative.

Key words: primordial germ cells, mouse embryo, histochemistry