用辣根过氧化物酶标记抗体Fab染色法在光镜及电镜下检测大肠癌的CEA标志

首都医科大学学报 ›› 1989, Vol. 10 ›› Issue (4): 264-267.

用辣根过氧化物酶标记抗体Fab染色法在光镜及电镜下检测大肠癌的CEA标志

李辉¹, 张惠琳², 堂道健一³, 长谷川³, 高天祥¹

1. 首都医学院;2. 山东医科大学;3. 日本东海大学

收稿日期:1989-01-16 修回日期:1900-01-01 出版日期:1989-10-15 发布日期:1989-10-15

Detection of CEA Marker of Colon-Rectal Carcinoma under Light and Electron Microscopes Using Horseradish Peroxidase Labeled Anti-CEA Fab Antibody

Li Hui¹

Laboratory of Electron Microscope, Capital Institute of Medicine

Received:1989-01-16 Revised:1900-01-01 Online:1989-10-15 Published:1989-10-15

摘要/Abstract

摘要： 采用辣根过氧化物酶标记的抗癌胚抗原(CEA)的抗体Fab片断,对4例大肠癌手术标本进行免疫组织化学染色,以检测肿瘤标志CEA。实验证明,此法简便易行、可靠,可用于检测消化系统的许多腺癌,做为病理诊断学的辅助手段。

关键词: 辣根过氧化物酶标记, 抗癌胚抗原(CEA)抗体, 免疫组织化学染色, 肿瘤标志CEA, 大肠癌

Abstract: Four specimens of colon-rectal carcinomas were stained histochemically and immunochemically using horseradish peroxidase labeled anti-CEA Fab antibody for the detection of CEA tumor marker, The results proved that Fab antibody fragment labeled with horseradish peroxidase better penetrated into the tissue owing to its smaller molecular weight, In addition, this method is simple, reliable and may be used for the detection of gastrointestinal adenocarcinomas as auxiliary procedure for pathological diagnosis.

Key words: horseradish peroxidase, antibody against CEA, immunohistochemical staining) tunot's market CEA, colon-tectal carcinoma

李辉;张惠琳;堂道健一;长谷川;高天祥. 用辣根过氧化物酶标记抗体Fab染色法在光镜及电镜下检测大肠癌的CEA标志[J]. 首都医科大学学报, 1989, 10(4): 264-267.

Li Hui. Detection of CEA Marker of Colon-Rectal Carcinoma under Light and Electron Microscopes Using Horseradish Peroxidase Labeled Anti-CEA Fab Antibody[J]. Journal of Capital Medical University, 1989, 10(4): 264-267.

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