关键词: 三带喙库蚊, 蚊, 细胞系, 细胞培养

Abstract: The establishment and growth of a cell line(CT-188) from fresh incubated larvae of Culex tritaeniorhynchus in a modified 199-medium is described. Only ethanol (70%)were used for sterilizing rather than the complex of mercuric chloride(HgCl₂) and ethanol. The majority of CT-188 cells were a fibroblast-type (approximately 95%); however, a small number of epithelial-like cells and other cells were also present in the culture. 10～11 days after the primary cells had covered the bottom of the container and could be subcultured. After seeding cells began to attach to the glass surface of the container within 30 minutes and reached the division peak in 48 hours. Most of the cells had the normal 2n chromosome complement of 6, although on occasion cells with more this number were seen. The cells are stored in liquid nitrogen(-196℃)at various passage levels and have been successfully resuscitated.

Key words: Culex tritaeniorhynchus, mosquito, cell lin, cell culture