STR分型技术鉴定后两株U87胶质瘤细胞系生物学特性评价

doi:10.3969/j.issn.1006-7795.2018.01.014

首都医科大学学报 ›› 2018, Vol. 39 ›› Issue (1): 74-78.doi: 10.3969/j.issn.1006-7795.2018.01.014

STR分型技术鉴定后两株U87胶质瘤细胞系生物学特性评价

张文丽¹, 孔凡虹¹, 何露¹, 董成亚², 王雅杰^2,3

1. 首都医科大学附属北京天坛医院检验科, 北京 100050;
2. 首都医科大学附属北京天坛医院临床医学研究实验室, 北京 100050;
3. 首都医科大学附属北京地坛医院检验科, 北京 100015

收稿日期:2017-07-19 出版日期:2018-01-21 发布日期:2018-01-27
通讯作者: 王雅杰 E-mail:tiantanwyj@aliyun.com
基金资助:
国家自然科学基金（81572474），北京市中医药科技发展资金项目（JJ2015-14）。

Evaluation of biological characteristics of two u87 glioma cell lines after STR typing technique

Zhang Wenli¹, Kong Fanhong¹, He Lu¹, Dong Chengya², Wang Yajie^2,3

1. Department of Clinical Laboratory, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China;
2. Laboratory of Clinical Medical Research, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;
3. Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China

Received:2017-07-19 Online:2018-01-21 Published:2018-01-27
Supported by:
This study was supported by National Natural Science Foundation of China (81572474),Science and Technology Development Fund Project of Traditional Chinese Medicine of Beijing (JJ2015-14).

摘要/Abstract

摘要： 目的对经过短串联重复序列（short tandem repeats，STR）分型检测技术鉴定后的两株U87胶质瘤细胞系[（美国典型培养物保藏中心（American Type Culture Collection，ATCC）数据库中U-87 MG Glioblastoma-Astrocytoma Human和德国微生物菌种保藏中心（Deutsche Sammlung von Mikroorganismen und Zellkulturen，DSMZ）数据库中U-87 MG]进行细胞生物学特性测定，对两株细胞用于后续研究工作的可能性进行评价。方法分别采用CCK-8法、划痕实验、Transwell实验等方法，并结合显微镜下细胞形态学特征，比较两株肿瘤细胞系的增生、迁移及侵袭能力等生物学特性。结果（1）CCK-8法：细胞生长曲线提示细胞增生能力差异无统计学意义（P>0.05）。（2）划痕实验：两细胞系迁移能力差异无统计学意义（P>0.05）。（3）Transwell实验：两细胞系迁移能力差异无统计学意义（P>0.05），DSMZ数据库中U-87 MG细胞侵袭能力高于ATCC数据库中U-87 MG Glioblastoma-AstrocytomaHuman细胞，差异有统计学意义（P<0.05）。结论两株U87细胞系均可反映脑胶质瘤的生物学特性，可用于后续科研实验。两细胞系的增生、迁移能力差异无统计学意义，侵袭能力差异具有统计学意义，可根据实验目的选择合适的细胞系。

关键词: 胶质瘤, U87细胞系, 生物学特性

Abstract: Objective To detect cell biological characteristics of the two U87 glioma cell lines[U-87 MG Glioblastoma-Astrocytoma Human in American Type Culture Collection (ATCC) database and U-87 MG in Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) database] which were identified by short tandem repeats (STR) typing technique, and to evaluate the possibility of two cells for follow-up research.Methods By CCK-8 method, scratch test, transwell assay, and combined with the morphological characteristics were done to investigate the proliferation, migration and invasion ability of the two tumor cell lines.Results (1) CCK-8 method:the cell growth curve showed no statistical difference in proliferation ability(P>0.05). (2) Scratch wound test:the migration ability of two cell lines is no statistical difference (P>0.05). (3) Transwell assay:the migration ability of two cell lines is no significant difference (P> 0.05), and the invasive ability of U-87 MG in DSMZ database is significantly higher than that of U-87 MG Glioblastoma-AstrocytomaHuman in ATCC database (P<0.05).Conclusion Both two U87 cell lines represent the biological properties of brain gliomas and can be used for subsequent experimental studies. The proliferation ability and migration ability of the two cell lines are not significantly different, and the difference of invasive ability is statistically significant. We can choose the appropriate cell line for experimental purposes.

Key words: glioma, U87 cell lines, biological characteristics

中图分类号:

R739.41

张文丽, 孔凡虹, 何露, 董成亚, 王雅杰. STR分型技术鉴定后两株U87胶质瘤细胞系生物学特性评价[J]. 首都医科大学学报, 2018, 39(1): 74-78.

Zhang Wenli, Kong Fanhong, He Lu, Dong Chengya, Wang Yajie. Evaluation of biological characteristics of two u87 glioma cell lines after STR typing technique[J]. Journal of Capital Medical University, 2018, 39(1): 74-78.

参考文献

[1] Morgan L L. The epidemiology of glioma in adults:a "state of the science" review[J]. Neuro-oncology, 2015,17(4):623-624.
[2] Ahmed R, Oborski M J, Hwang M, et al. Malignant gliomas:current perspectives in diagnosis, treatment, and early response assessment using advanced quantitative imaging methods[J]. Cancer Manag Res, 2014, 6:149-170.
[3] 李连进, 佟建洲,崔敬,等.脑胶质瘤组织AKT和PTEN的测定意义[J].中华肿瘤防治杂志,2016,23(S1):24-25.
[4] 李岩, 石蕊,陈建新,等.VM-26和DDP联合化疗对复发高级别脑胶质瘤的治疗体会[J].中华肿瘤防治杂志,2015,22(5):786-790.
[5] Xie Q, Mittal S, Berens M E. Targeting adaptive glioblastoma:an overview of proliferation and invasion[J]. Neuro Oncol, 2014, 16(12):1575-1584.
[6] Kanno H, Sato H, Yokoyama T A, et al. The VHL tumor suppressor protein regulates tumorigenicity of U87-derived glioma stem-like cells by inhibiting the JAK/STAT signaling pathway[J]. Int J Oncol, 2013, 42(3):881-886.
[7] Barallon R, Bauer S R, Butler J, et al. Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues[J]. In Vitro Cell Dev Biol Anim,2010,46(9):727-732.
[8] Masters, J R. Cell-line authentication:End the scandal of false cell lines[J]. Nature, 2012, 492(7428):186.
[9] Dirks W G, Drexler H G. STR DNA typing of human cell lines:detection of intra-and interspecies cross-contamination[J]. Methods Mol Biol,2013,946:27-38.
[10] 张文丽,孔凡虹,贺文艳,等.细胞培养实验中细胞系鉴定及质量控制重要性探讨[J]. 标记免疫分析与临床, 2017, 24(1):84-87.
[11] Masters, J R. Cell-line authentication:End the scandal of false cell lines[J]. Nature, 2012, 492(7428):186.
[12] Lazebnik Y. What are the hallmarks of cancer?[J]. Nat Rev Cancer, 2010, 10(4):232-233.
[13] Hanahan D, Weinberg R A. Hallmarks of cancer:the next generation[J]. Cell, 2011,144(5):646-674.
[14] 王维娜,梁月勉,赵春兰.多灶性脑胶质瘤伴肺转移一例报告[J].中华肿瘤防治杂志,2015,22(4):312-314.

[1]	张姝, 王凯, 杨子皓, 乔真, 李晓桐, 袁磊磊, 艾林. ¹¹C-MET PET/CT筛查幕上较低级别胶质瘤IDH和1p/19q分子分型的应用价值[J]. 首都医科大学学报, 2022, 43(6): 826-833.
[2]	徐洋, 王凯, 陈嫱, 艾林. 核医学分子影像在胶质瘤诊疗中的研究进展[J]. 首都医科大学学报, 2022, 43(6): 880-885.
[3]	闫新亭, 宋双双, 卢洁. 脑胶质瘤表观扩散系数与肿瘤分级及IDH1突变的关系[J]. 首都医科大学学报, 2021, 42(2): 286-292.
[4]	江涛. 脑胶质瘤精准医疗——江涛教授[J]. 首都医科大学学报, 2020, 41(5): 854-859.
[5]	孙梦雪, 王雷明, 滕梁红. 胶质瘤中BRAF基因异常的研究进展[J]. 首都医科大学学报, 2020, 41(3): 380-384.
[6]	陈峰, 郑晓红, 李文斌. 脑胶质瘤免疫治疗进展[J]. 首都医科大学学报, 2019, 40(6): 966-971.
[7]	王田田, 高琛琛, 李利生, 徐敬东. 消化道巨噬细胞的功能与炎性肠病和肠道肿瘤的相关性研究进展[J]. 首都医科大学学报, 2019, 40(6): 972-981.
[8]	于春娜, 江波, 李文斌, 陈峰. β-羟丁酸对脑胶质瘤细胞增生糖酵解的影响[J]. 首都医科大学学报, 2019, 40(2): 186-190.
[9]	张传宝, 江涛. 脑胶质瘤诊疗关键技术创新与推广应用——2017年度国家科学技术进步二等奖[J]. 首都医科大学学报, 2018, 39(1): 1-5.
[10]	李岩, 石蕊, 陈建新, 李珊, 康勋, 陈静, 赵凯红, 李文斌. VM-26和DDP联合化疗对复发高级别脑胶质瘤的治疗体会[J]. 首都医科大学学报, 2015, 36(5): 786-790.
[11]	邢天禹, 牛静, 丁卫. 聚二甲基硅氧烷定制微培养装置及其在肿瘤细胞迁移浸润动态分析中的应用[J]. 首都医科大学学报, 2015, 36(2): 270-275.
[12]	张露勇, 罗菲亚, 胡培丽, 单纯, 张淼. 神经酰胺通过JNK-c-Jun信号通路诱导胶质瘤细胞自噬性死亡[J]. 首都医科大学学报, 2015, 36(2): 276-281.
[13]	邢树山, 胡万宁, 赵喜庆, 刘文庆, 宫剑. 白介素1受体辅助蛋白在儿童和成人低级别胶质瘤中的作用[J]. 首都医科大学学报, 2013, 34(2): 258-263.
[14]	康勋;李珊;谢铮铮;赵艳杰;姜妮;周蕾;李文斌. 恶性脑胶质瘤同步放化疗中替莫唑胺化疗的不良反应及对策[J]. 首都医科大学学报, 2012, 33(5): 589-593.
[15]	陆峥;廖浩;谢坚. 额极胶质瘤扩大切除方法及疗效分析[J]. 首都医科大学学报, 2010, 31(5): 653-656.

STR分型技术鉴定后两株U87胶质瘤细胞系生物学特性评价

Evaluation of biological characteristics of two u87 glioma cell lines after STR typing technique

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价