一种新的血管紧张素I转换酶抑制剂八肽与P物质3-4对人转换酶抑制作用的研究

首都医科大学学报 ›› 1990, Vol. 11 ›› Issue (4): 279-282.

一种新的血管紧张素I转换酶抑制剂八肽与P物质3-4对人转换酶抑制作用的研究

郭春远¹, 刘传弟¹, 盛树力², 刘爱民², 陈雁江², 胡晓愚³

1. 首都医学院宣武医院;2. 北京市神经外科研究所;3. 兰州大学

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1990-10-15 发布日期:1990-10-15

Biolgical Activity of a New AngiotensinⅠConverting Enzyme Inhibitor and a N-terminal Fragment of Substance P

Guo Chunyuan¹, Liu Chuandi¹, Sheng Shuli², Liu Aimin², Chen Yanjiang², Hu xiaoyu³

Received:1900-01-01 Revised:1900-01-01 Online:1990-10-15 Published:1990-10-15

摘要/Abstract

摘要： 用固相法合成了一种新的肽类血管紧张素Ⅰ转换酶抑制剂(AI,Pro-Thr-His-Ile-Lys-TrpGly-Asp)和P物质N末端片段(SP3-4,Gly-Phy),并检测了AI和SP3-4对人血浆、脑和肺组织中血管紧张素Ⅰ转换酶(ACE)的抑制作用,结果表明:①合成的AI和SP3-4经HPLC分离鉴定纯度均为>95%,②AI抑制人血浆(IC50 19±4.6/μmol/L)脑(IC50 26±6.3μmol/L)和肺(2例,IC50 119～14.1μmol/L)中ACE活性,SP3-4对人体内ACE活性无抑制。

关键词: 血管紧张素Ⅰ转换酶抑制剂, P物质片段, 血管紧张素I转换酶

Abstract: A new angiotensin I converting enzyme inhibitor(AI, Pro-Thr-His-Ile-Lys Trp-Gly-Asp) and a N-terminal fragment of substance P (SP3-4, Gly-Phy) were synthesized by solid phase method. Their capacity to inhibit angiotensin I converting enzyme (ACE) seperated from human blood, brain and lung was tested by biochemical assay. Results showed that 1. All of the synthetic peptides were_assayed and their purity was>95% as established by HPLC analysis. 2. The AI inhibited the ACE from human blood(IC50 19±4.6μmol/L) brain (IC50 26±6.3μmol/L) and lung (2 cases, IC5011.9～14.1μmol/L) but the SP3-4 failed to inhibited the ACE from human being.

Key words: dangiotensin I converting enzyme inhinsin biotor, N-terminal fragment of substance P, anigiotensin converting enzyme

郭春远;刘传弟;盛树力;刘爱民;陈雁江;胡晓愚. 一种新的血管紧张素I转换酶抑制剂八肽与P物质3-4对人转换酶抑制作用的研究[J]. 首都医科大学学报, 1990, 11(4): 279-282.

Guo Chunyuan;Liu Chuandi;Sheng Shuli;Liu Aimin;Chen Yanjiang;Hu xiaoyu. Biolgical Activity of a New AngiotensinⅠConverting Enzyme Inhibitor and a N-terminal Fragment of Substance P[J]. Journal of Capital Medical University, 1990, 11(4): 279-282.