斑点-ELISA与平板-ELISA诊断脑囊虫病的敏感性比较观察

首都医科大学学报 ›› 1991, Vol. 12 ›› Issue (4): 257-259.

斑点-ELISA与平板-ELISA诊断脑囊虫病的敏感性比较观察

诸欣平, 卞英华, 姜洪杰

首都医学院寄生虫学教研室

收稿日期:1991-04-13 修回日期:1900-01-01 出版日期:1991-10-15 发布日期:1991-10-15

A Comparative Study of Diagnosis for Cerebrocysticercosis by Dot-ELISA and Standard ELISA

Zhu Xinping, Bian Yinghua, Jiang Hongjie

Department of Parasitology, Capital Institute of Medicine

Received:1991-04-13 Revised:1900-01-01 Online:1991-10-15 Published:1991-10-15

摘要/Abstract

摘要： 应用斑点-ELISA和平板-ELISA对52例脑囊虫病患者的血清进行抗体检测。结果显示,两种免疫方法诊断脑囊虫病的敏感性近似(阳性率分别为92.3%与90.4%);与40例正常人血清均无假阳性反应。但斑点-ELISA具有节省抗原,操作简便,不需特殊仪器设备等优点,适用于临床诊断及流行病学调查。

关键词: 斑点-ELISA, 平板-ELISA, 脑囊虫病

Abstract: The dot enzyme-linked immunosorbent assay(Dot-ELISA)and standard enzyme-linked immunosorbent assay(ELISA)were used to detect Cysticercus cellulosae antibodies in the sera of the patients with cerebroeysticercosis.By using Dot-ELISA,47 out of 52 pa-tients(92.3%)showed a positive reaction.In the standard ELISA,47 out of 52 patients (90.4%)showed a positive reaction.None of the 40 serum samples from normal controls was found positive by both assays.Although there were similar higher sensitivity and speci-ficity in the diagnosis of cerebrocysticereosis by both Dot-ELISA and standard ELISA,the Dot-ELISA is more economical,simple and do not need any specific equipment for the diagnosis of cerebrocysticercosis.Therefore,Dot-ELISA is suitable for clinic application and epidemic survey.

Key words: Dot-ELISA, ELISA, cerebrocysticercosis

诸欣平;卞英华;姜洪杰. 斑点-ELISA与平板-ELISA诊断脑囊虫病的敏感性比较观察[J]. 首都医科大学学报, 1991, 12(4): 257-259.

Zhu Xinping;Bian Yinghua;Jiang Hongjie. A Comparative Study of Diagnosis for Cerebrocysticercosis by Dot-ELISA and Standard ELISA[J]. Journal of Capital Medical University, 1991, 12(4): 257-259.

[1]	郭冬梅;谢淑萍;刘爱华. 6585例脑囊虫病的分型及其头痛的分析[J]. 首都医科大学学报, 2001, 22(4): 349-351.
[2]	温艳;张月清. 检测受染小鼠血清中循环抗原以诊断旋毛虫病的研究[J]. 首都医科大学学报, 1990, 11(1): 22-27.