凝胶色谱法研究配体与人血清蛋白质竞争铍的作用

首都医科大学学报 ›› 1993, Vol. 14 ›› Issue (2): 106-111.

凝胶色谱法研究配体与人血清蛋白质竞争铍的作用

张锦楠, 曾昭辉

首都医学院化学教研室

收稿日期:1992-03-22 修回日期:1900-01-01 出版日期:1993-04-15 发布日期:1993-04-15

Study on the Competition of Ligans with Human Serum Proteins for Be(Ⅱ) by Gel Chromatography

Zhang Jinnan, Zeng Zhaohui

Department of Chemistry, Beijing Capital Institute of Medicine

Received:1992-03-22 Revised:1900-01-01 Online:1993-04-15 Published:1993-04-15

摘要/Abstract

摘要： 用凝胶色谱法和原子吸收分光光度法.研究了有毒金属铍与人血清蛋白质的结合情况,及20种配体与蛋白质竞争铍的作用情况。结果发现:①在生理 pH 条件下人血清蛋白质能与铍强烈地结合,平衡时被蛋白质结合的铍量达95%,②有几种配体能使结合在蛋白质大分子上的铍下降到78%～18%,成为镀的小分子配体络合物。并且通过结构上分子基团的变换与竞争能力的比较,发现了有竞争能力的配体分子在结构上的规律性。

关键词: 有毒金属铍(Be), 凝胶色谱法, 金属促排络合剂, 人血清蛋白质

Abstract: The binding of Be(Ⅱ) to human serum proteins and the competition of 20 ligands with proteins for Be(Ⅱ) were studied by gel chromatography and atomic absorption spec-trophotometry.The results showed that ①Be(Ⅱ) was bound by serum protein strongly un-der physiological pH condition and the binding rate was about 95% at equilibuium condition. ②Among these ligand studed,several ligands could compete effectively with serum protein for Be(Ⅱ) and about 78%～18% of Be(Ⅱ) may thus be removed from proteins and form complex of small molecular ligand of Be(Ⅱ).The structural regularity for the carboxylic acid ligands was found through changing substitution groups and compairing their competi-tive capacity.

Key words: toxic metal beryllium Be(Ⅱ), gel chromatography, complexing agents for removing metals human serum proteins

中图分类号:

Q614.21

张锦楠;曾昭辉. 凝胶色谱法研究配体与人血清蛋白质竞争铍的作用[J]. 首都医科大学学报, 1993, 14(2): 106-111.

Zhang Jinnan;Zeng Zhaohui. Study on the Competition of Ligans with Human Serum Proteins for Be(Ⅱ) by Gel Chromatography[J]. Journal of Capital Medical University, 1993, 14(2): 106-111.