人类乳头瘤病毒16型DNA诱导NIH　3T3细胞的恶性转化

首都医科大学学报 ›› 1994, Vol. 15 ›› Issue (2): 109-111.

人类乳头瘤病毒16型DNA诱导NIH　3T3细胞的恶性转化

盖其伟¹, 赫翠珍¹, 姚庆英¹, 高天祥¹, 周利华², 刘晓明²

1. 首都医学院电镜室;2. 山东医科大学电镜室

收稿日期:1993-04-02 修回日期:1900-01-01 出版日期:1994-04-15 发布日期:1994-04-15

Human Papilloma Virus Type 16 DNA Induces Malignant Transformation of NIH 3T3 Cells

Gai Qiwei¹, He Cuizhen¹, Yao Qingying¹, Gao Tienxiang¹, Zhou Lihua², Liu Xiaoming²

1. Laboratory of Electron Microscopy, Capital Institute of Medicine;2. Laboratory of Electron Microscopy, Shandong Medical University

Received:1993-04-02 Revised:1900-01-01 Online:1994-04-15 Published:1994-04-15

摘要/Abstract

摘要： 用人类乳头瘤病毒(human papillomavirus,HPV)16型DNA,通过磷酸钙沉淀技术在体外成功地使NIH3T3细胞发生恶性转化,转化细胞克隆后分析表明存在如下特征:转化细胞形态上变宽,呈多角状生长并形成集落;细胞的停泊依赖性下降;能在含1%小牛血清培养基中良好生长.转化细胞的密度依赖性下降.用Southernblot杂交发现HPV16DNA以整合状态存在于NIH3T3细胞DNA中.

关键词: 人类乳头瘤病毒(HPV), NIH3T3细胞, 恶性转化

Abstract: Using calcium phosphate precipitation technique,we transformed in vitro NIH cell line successfully with human papilloma virus(HPV)type 16 DNA. Further analysis showed that the transformed cells possessed refractile,roundish,pile-up growth morphology,and decrease of anchorage dependence(growth in semi-fluid agar),serum dependence, and density dependence.Southern blot hybridization of transformed cell DNA indicated that HPV-16 DNA was incorporated into NIH 3T3 cells. The results indicate that HPV-16 DNA is responsible for the transformation of NIH 3T3 cells.

Key words: human papilloma virus, NIH 3T3 cell, malignant transformation

中图分类号:

R373

盖其伟;赫翠珍;姚庆英;高天祥;周利华;刘晓明. 人类乳头瘤病毒16型DNA诱导NIH　3T3细胞的恶性转化[J]. 首都医科大学学报, 1994, 15(2): 109-111.

Gai Qiwei;He Cuizhen;Yao Qingying;Gao Tienxiang;Zhou Lihua;Liu Xiaoming. Human Papilloma Virus Type 16 DNA Induces Malignant Transformation of NIH 3T3 Cells[J]. Journal of Capital Medical University, 1994, 15(2): 109-111.

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