关键词: 咽喉上皮增生性病变, 人类乳头瘤病毒, 免疫组织化学, 聚合酶链反应

Abstract:

We assayed HPV-Ag and HPVDNA using immunohistochemical stain and PCR amplification respectively in 51 laryngopharyngeal epithelial hyperplastic lesions and correlated these findinfg with histological changes.The group consists of 37 laryngopharyngeal papillomatous lesions, 14 squamous carcinoma of the larynx.We reclassfied into 3 subgroups : 9 cases of condyloma acuminata like lesion, 18 cases of notype condyloma acuminata like lesion,and 10 cases of squamous cell papillomas.In the subgroup of condyloma acuminata like lesion, the incidence of HPV-Ag positive immunohistochemical stain was 33% while,it is 0% in the other two subgroups.In the first two subgroups the HPV-DNA by PCR amplification was manifested in 100% cases, but it was detected only in 10% in the third subgroup.14 squamous carcinoma of the larynx was composed of 4 verrucous carcinoma and 10 well differentiated squamous cell carcinoma.The HPVAg was detected immunohistochemically in 10%(1/10) of well differentiated squamous cell carcinoma while it was 0% in verrucous carcinoma.In addition to this findings.HPV-DNA was detected in 50% (2/4) of verrucous carcinoma and 30% (3/10) of well differentiated squamous cell carcinoma.Our results suggestedthat the development of some subgroups of the laryngopharyngeal epithelial hyperplastic lesions was related to the infection of HPV.We put foward of the condyloma acuminate of the larynx is a pathological entity.We believe PCR amplification is the most sensitive technique for detection of HPV.

Key words: laryngopharyngeal epithelial hyperplastic lesions, human papillomavirus, immunohistochemistry, polymerase chain reaction