聚合酶链反应在诊断肺结核中的应用

首都医科大学学报 ›› 1998, Vol. 19 ›› Issue (1): 31-33.

聚合酶链反应在诊断肺结核中的应用

刘双¹, 姚天樵¹, 徐利¹, 祁雅慧², 殷红², 姚庆英², 赫翠珍², 高天祥²

1. 首都医科大学附属北京安贞医院呼吸内科;2. 首都医科大学实验中心

收稿日期:1997-03-15 修回日期:1900-01-01 出版日期:1998-01-15 发布日期:1998-01-15

Polymerase Chain Reaction for the Diagnosis of Pulmonary Tuberculousis

Liu Shuang¹, Yao Tianqiao¹, Xu Li¹, Qi Yahui², Yin Hong², Yao Qingying², He Cuizhen², Gao Tianxiang²

1. Department of Chest Medicine, Beijing Anzhen Hospital, Affiliate of Capital University of Medical Sciences;2. Laboratory Center, Capital University of Medical Sciences

Received:1997-03-15 Revised:1900-01-01 Online:1998-01-15 Published:1998-01-15

摘要/Abstract

摘要： 应用聚合酶链反应(PCR)技术对78例患者的痰、支气管肺泡灌洗液(BALF)和胸水标本进行结核菌检测;并同时进行涂片抗酸染色、皮肤结核菌素试验及血结核菌抗体检测.结果:PCR阳性率为46.15%,与涂片镜检、结核菌素试验强阳性、血结核菌抗体阳性率(分别为3.85%、17.95%、11.54%)相比均有显着性差异(P<0.05).由此可见PCR方法比传统方法具有更高的结核杆菌检出率.

关键词: 聚合酶链反应, 结核杆菌, 肺结核

Abstract: Polymerase chain reaction(PCR)was applied to detect DNA of mycobacterium tuberculosis(TB)in 78 clinical samples. Methods: For each sample, TB-PCR, the positive rate of acid fast staining, tuberculin skin test and tuberculous antibody were made. The results of PCR were compared with those of conventional procedures and clinical diagnosis standard. Results: It was found that the positive rate of acid-fast staining, tuberculin skin test, tuberculous antibody and PCR were 3.85%、17.95%,11.54% and 46.15% respectively in 78 tuberculous samples. The sensitivity of PCR was much higher than the former diagnostic procedures. Conclusions: up to now, the results showed that the PCR was a valuable detective tool for diagnosis and differential diagnosis of tuberculosis. Diagnosis of tuberculousis still need to be determined by the patient's chlinical menfestions and others methods.

Key words: polymerase chain reaction(PCR), mycobacterium tuberculosis, pulmonary tuberculousis

中图分类号:

R521

刘双;姚天樵;徐利;祁雅慧;殷红;姚庆英;赫翠珍;高天祥. 聚合酶链反应在诊断肺结核中的应用[J]. 首都医科大学学报, 1998, 19(1): 31-33.

Liu Shuang;Yao Tianqiao;Xu Li;Qi Yahui;Yin Hong;Yao Qingying;He Cuizhen;Gao Tianxiang. Polymerase Chain Reaction for the Diagnosis of Pulmonary Tuberculousis[J]. Journal of Capital Medical University, 1998, 19(1): 31-33.

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