去甲斑蝥素用于单克隆抗体为载体的肿瘤导向治疗实验研究

首都医科大学学报 ›› 1999, Vol. 20 ›› Issue (1): 5-8.

去甲斑蝥素用于单克隆抗体为载体的肿瘤导向治疗实验研究

梁福佑¹, 张胜华², 潘颖¹, 徐琳娜², 王维刚²

1. 首都医科大学细胞生物学研究室;2. 中国医学科学院中国协和医科大学医药生物技术研究所

收稿日期:1998-12-16 修回日期:1900-01-01 出版日期:1999-01-15 发布日期:2013-10-16

Use of Monoclonal Antibody Norcantharidin Conjugate in Experimental Targeting Therapy of Tumor

Liang Fuyou¹, Zhang Shenghua², Pan Ying¹, Xu Linna², Wang Weigang²

1. Department of Cell Biology, Capital University of Medical Sciences;2. Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College

Received:1998-12-16 Revised:1900-01-01 Online:1999-01-15 Published:2013-10-16

摘要/Abstract

摘要： 用单抗3A5制备与去甲斑蝥素(NCTD)的偶联物。ELISA检测结果表明:3A5-NCTD偶联物保持对人肝癌BEL-7402细胞的免疫反应性。克隆生成法测定结果显示:3A5-NCTD具有比NCTD更强的细胞毒性,两者的IC₅₀分别为2.3mg/L和4.2mg/L.小鼠腹腔内移植H22肝癌,腹膜内给药(ip),3A5-NCTD(3mg/kg)和NCTD(3mg/kg)的延长寿命值(ILS)分别为168%和42%.裸鼠移植人肝癌BEL-7402细胞,静脉给药(iv),NCTD的肿瘤抑制率为15%(P>0.05),3A5-NCTD为62%(P<0.01);瘤结周围给药(pt),NCTD的肿瘤抑制率为47%(P<0.05),3A5-NCTD为78%(P<0.01).结果表明,与NCTD相比,3A5-NCTD对裸鼠移植人肝癌和腹腔肿瘤具有更强的治疗作用。以上结果提示单抗与NCTD偶联物在肿瘤的导向治疗中有较好的疗效。

关键词: 单克隆抗体, 去甲斑蝥素, 免疫偶联物

Abstract: Arat monoclonal antibody was linked to norcantharidin (NCTD), an antitumor drug currently in clinical use McAb 3A5. The 3A5-NCTD conjugate retained complete immunoreactivity of McAb 3A5 to human hepatoma cancer BEL-7402 cells. The IC₅₀ values of 3A5-NCTD and free NCTD were 2.3 mg/Land 4.2 mg/L, respectively. The 3A5-NCTD showed higher cytotoxicity than free NCTD. Hepatoma H22 ascites was transplanted into the peritoneal of mice. 3A5-NCTD or NCTD were injected into the cavity. The ILS(%) values were 168% and 42%. Tumor fragments of human hepatoma BEL-7402 were transplanted into nude mice. Then 3A5 NCTD or NCTD was injected iv, or pt(peritumorally). The inhibition rates on the growth of hepatoma BEL-7402 xenografts were 62% and 15% for iv; 78% and 47% for pt.Results indicate that 3A5-NCTD is highly effective against targeted human cancer xenograft and mouse tumor.

Key words: monoclonal antibody, norcantharidin, immunoconjugate

中图分类号:

R730.59

梁福佑;张胜华;潘颖;徐琳娜;王维刚. 去甲斑蝥素用于单克隆抗体为载体的肿瘤导向治疗实验研究[J]. 首都医科大学学报, 1999, 20(1): 5-8.

Liang Fuyou;Zhang Shenghua;Pan Ying;Xu Linna;Wang Weigang. Use of Monoclonal Antibody Norcantharidin Conjugate in Experimental Targeting Therapy of Tumor[J]. Journal of Capital Medical University, 1999, 20(1): 5-8.

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