日本血吸虫感染小鼠脾细胞体外IL-2转录水平的实验观察

首都医科大学学报 ›› 2000, Vol. 21 ›› Issue (2): 101-103.

日本血吸虫感染小鼠脾细胞体外IL-2转录水平的实验观察

田小军¹, 薛燕萍¹, 诸欣平², 黄敏君¹

1. 北京热带医学研究所;2. 首都医科大学

收稿日期:1999-12-29 修回日期:1900-01-01 出版日期:2000-04-15 发布日期:2000-04-15

Experimental Observation of in Vitro IL-2 Transcription on the Spleen Cells of Mice Infected by Schistosoma japonicum

Tian Xiaojun¹, Xue Yanping¹, Zhu Xinping², Huang Minjun¹

1. Beijing Tropical Medicine Research Institute;2. Capital University of Medical Sciences

Received:1999-12-29 Revised:1900-01-01 Online:2000-04-15 Published:2000-04-15

摘要/Abstract

摘要： 应用RT-PCR方法对日本血吸虫感染小鼠脾细胞体外在SEA与ConA诱导下产生的IL-2从mRNA转录水平进行研究，以探讨该细胞因子mRNA转录水平的动态变化及其在肉芽肿形成与调节过程中的作用。结果显示，未感染和感染3周小鼠脾细胞均无IL-2表达，感染后第5周的小鼠脾细胞出现IL-2特异性条带，感染后第8周IL-2表达明显增强，感染后第10、12周无论SEA或ConA都不能诱导IL-2 mRNA的转录。表明IL-2表达的动态变化与肉芽肿的形成、发展及调节相平行，提示IL-2可能在肉芽肿形成和调节过程中起重要作用。

关键词: 日本血吸虫, 可溶性虫卵抗原, 白细胞介素2, 逆转录聚合酶链反应

Abstract: In the present study, IL-2 mRNA levels was observed on the spleen cells of mice infected with Schistosoma japonicum by RT-PCR in order to find out how relevant the dynamics of the gene expression of the cytokines to granulomatous inflammation. It was found that the expression of IL-2 could not be detected in SEA or ConA treated spleen cells from both uninfected and 3 week infected mice. IL-2 mRNA could be found obviously expressed at 5 week infection, significantly increased at 8 week infection, However, neither 10 and 12 infected mice indicating the modulated expression of mRNA of the cytokine. The observation revealed that the dynamic of IL-2 gene expression paralleled to the granuloma formation and modulation in S. japonicum infected BALB/c mice and that IL-2 may play an important role in the modulation of granuloma formation in schistosomiasis japonica .

Key words: Schistosoma japonicum, soluble egg antigen, Interleukin-2, RT-PCR

中图分类号:

R383.2

田小军;薛燕萍;诸欣平;黄敏君. 日本血吸虫感染小鼠脾细胞体外IL-2转录水平的实验观察[J]. 首都医科大学学报, 2000, 21(2): 101-103.

Tian Xiaojun;Xue Yanping;Zhu Xinping;Huang Minjun. Experimental Observation of in Vitro IL-2 Transcription on the Spleen Cells of Mice Infected by Schistosoma japonicum[J]. Journal of Capital Medical University, 2000, 21(2): 101-103.

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