中药复方962对老龄大鼠皮质胆碱乙酰转移酶和乙酰胆碱酯酶活性的影响

首都医科大学学报 ›› 2000, Vol. 21 ›› Issue (3): 190-192.

中药复方962对老龄大鼠皮质胆碱乙酰转移酶和乙酰胆碱酯酶活性的影响

李海玲¹, 李林¹, 李斌¹, 叶翠飞¹, 李宗信¹, 何士大¹, 左萍萍²

1. 首都医科大学宣武医院北京市脑老化重点实验室;2. 首都医科大学宣武医院北京市脑老化重点实验

收稿日期:1999-10-22 修回日期:1900-01-01 出版日期:2000-07-15 发布日期:2000-07-15

Effects of Traditional Chinese Compound 962 on Choline Acetyltransferase and Acetylcholinesterase in Cerebral Cortex of Aged Rats

Li Hailing¹, Li Lin¹, Li Bin¹, Ye Cuifei¹, Li Zongxin¹, He Shida¹, Zuo Pingping²

1. Beijing Key Laboratory for Brain Aging, Xuanwu Hospital, Affiliate of Capital University of Medical Sciences;2. Chinese Academy of Medical Sciences

Received:1999-10-22 Revised:1900-01-01 Online:2000-07-15 Published:2000-07-15

摘要/Abstract

摘要： 为研究中药复方962胶囊对老龄大鼠皮质胆碱乙酰转移酶(ChAT)和乙酰胆碱酯酶(AchE)活性的影响,将22月龄和24月龄Wistar大鼠分为青年对照组、老年模型组、阳性对照脑复康组(0.56g/kg)、962中剂量组(0.9g/kg)和962大剂量组(1.8g/kg)5组,分别灌胃给药1～2个月后处死大鼠。用Fonnun法和羟胺比色法分别测定5组大鼠皮质的ChAT和AchE的活性。结果：中药复方962大剂量(1.8g/kg)对老龄大鼠皮质ChAT活性有提高作用(P<0.05),但对AchE无显着影响。提示：中药复方962可能通过影响老龄大鼠中枢胆碱能神经系统而改善其衰老的变化.

关键词: 中药复方962, 老龄大鼠, 胆碱乙酰转移酶, 乙酰胆碱酯酶

Abstract: In order to determine the effect of the compound 962 capsule on the choline acetyltransferase and acetylcholinesterase in cerebral cortex of aged rats, and to probe the anti agedness mechanism from the view of choline energy nerve. Male and female aged rats(22-month old and 24-month old) were classified into five groups young——control group, old model group, piracetem group (0.56 g/kg), 962 middle dose group (0.9 g/kg) and 962 high dose group (1.8 g/kg). All test drug was administrated for 1～2 months by ig. The choline acetyltransferase and acetylcholinesterase activity were measured by Fonnun and hydroxylamine colorimetry method. Results: Compound 962 improved the activity of choline acetyltransferase (P< 0.05), but it had little effect on the acetylcholinesterase in cerebral cortex of aged rats. This indicated that by affecting the central nervous system in aged rats, compound 962 capsule could improve the aging process.

Key words: traditional Chinese compound 962, aged rat, choline acetyltransferase, acetylcholinesterase

中图分类号:

R285.5
R592

李海玲;李林;李斌;叶翠飞;李宗信;何士大;左萍萍. 中药复方962对老龄大鼠皮质胆碱乙酰转移酶和乙酰胆碱酯酶活性的影响[J]. 首都医科大学学报, 2000, 21(3): 190-192.

Li Hailing;Li Lin;Li Bin;Ye Cuifei;Li Zongxin;He Shida;Zuo Pingping. Effects of Traditional Chinese Compound 962 on Choline Acetyltransferase and Acetylcholinesterase in Cerebral Cortex of Aged Rats[J]. Journal of Capital Medical University, 2000, 21(3): 190-192.

[1]	张悦, 郑丽飞, 宋瑾, 樊瑞芳, 陈长亮, 朱进霞. 双侧黑质注射脂多糖引起大鼠迷走神经背核胆碱能标志物降低和便秘[J]. 首都医科大学学报, 2013, 34(4): 566-571.
[2]	闫颖, 李森, 赵志炜, 赵咏梅. β淀粉样肽结合乙醇脱氢酶及胆碱乙酰转移酶在糖尿病小鼠海马内表达的时程变化[J]. 首都医科大学学报, 2013, 34(1): 53-57.
[3]	王玉梅;曹凯;刘永平;秦博文;商亚珍. 黄芩茎叶黄酮对大鼠缺血性记忆障碍的改善作用及对胆碱乙酰转移酶和一氧化氮合酶蛋白表达的影响[J]. 首都医科大学学报, 2011, 32(4): 494-500.
[4]	王宪玲;贾建平;左萍萍;杨楠. 自发性2型糖尿病小鼠脑组织胆碱能神经递质系统的研究[J]. 首都医科大学学报, 2006, 27(3): 355-357.
[5]	纪中启;徐群渊;张进路;罗英瑾;李楠. 乙酰胆碱酯酶在家兔小脑内的分布[J]. 首都医科大学学报, 1992, 13(3): 165-170.