对羟苯甲酸3-羟化酶初步纯化及进化起源探讨

首都医科大学学报 ›› 2002, Vol. 23 ›› Issue (2): 101-104.

对羟苯甲酸3-羟化酶初步纯化及进化起源探讨

陈瑞¹, 细川桂一²

1. 首都医科大学生物化学教研室;2. 日本食品综合研究所微生物机能工学研究室

收稿日期:2001-11-20 修回日期:1900-01-01 出版日期:2002-04-15 发布日期:2002-04-15

Preliminary Purification and Pursuit of Evolutionary Origin of P-hydroxybenzoate 3-hydroxylase from Comamonas Testosteroni

Chen Rui¹, Hosokawa Keiichi²

1. Department of Biochemistry, Capital University of Medical Sciences;2. National Food Research Institute, Ministry of Agriculture Forestry and Fisheries

Received:2001-11-20 Revised:1900-01-01 Online:2002-04-15 Published:2002-04-15

摘要/Abstract

摘要： 经超声破碎、硫酸铵分级沉淀、凝胶过滤、离子交换层析和SDS-PAGE,从Comamonastestosteroni菌株中获得了相对分子质量为4.5万的对羟苯甲酸3-羟化酶(POB3-HOase);测定了其N-末端21个氨基酸序列,并与文献报道来源于不同菌株的POB3-HOase序列进行了同源性比较,结果提示它们具有相同的进化起源。

关键词: 对羟苯甲酸3-羟化酶, 纯化, 进化起源, 丛毛单孢菌

Abstract: The P-hydroxybenzoate 3-hydroxylase(POB-HOase)with 45 thousand relative molecular mass from Comamonas testosteroni was purified by using sonic crushing, ammonium sulfate fractionation, molecular sieve chromatography, exchange chromatography and SDS-PAGE. The N-termina lamino acid sequence was determined up to 21 residues. The amino acid sequence was compared with the known N-terminal sequences of POB3-HOases from Pseudomonas aeruginosa, Pseudomonas fluorescens and Acinetobacter calcoaceticus. The high degree of homology observed between the POB3-HOases from Comamonas and those of Pseudomonas and Acinetobacter indicates the common evolutionary origin of POB3-HOase among these soil bacteria of different genera.

Key words: para-hydroxybenzoare 3-hydroxylase|purification|evolutionary origin|Comamonas testosteroni

中图分类号:

Q554⁺.7

陈瑞;细川桂一. 对羟苯甲酸3-羟化酶初步纯化及进化起源探讨[J]. 首都医科大学学报, 2002, 23(2): 101-104.

Chen Rui;Hosokawa Keiichi. Preliminary Purification and Pursuit of Evolutionary Origin of P-hydroxybenzoate 3-hydroxylase from Comamonas Testosteroni[J]. Journal of Capital Medical University, 2002, 23(2): 101-104.

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