关键词: 定点突变, pBV-BPI₆₀₀-Fcγ1₇₀₀重组表达载体, BPI₂₃-Fcγ1重组抗菌蛋白

Abstract: In order to improve the expression and renaturation yield of BPI₂₃-Fcγ1 bactericidal recombinant protein in Prokaryotic system, pBV-BPI₆₀₀-Fcγ1₇₀₀ recombinant expression vector was reconstructed by site-directed mutation method. The mutational vector was transformed into the competent Escherichia coli DH5α and expressed by temperature induced method. The results showed the amount of the mutational BPI₂₃-Fcγ1 protein expression was 10% more than that of the non-mutant one, and its expression time was an hour earlier than the latter. Its bactericidal capability was the same as before, but its renaturation yield was not improved observably.

Key words: site-directed mutagenesis, pBV-BPI₆₀₀-Fcγ1₇₀₀ recombinant expression vector, BPI₂₃-Fcγ1 recombinant bactericidal protein