首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (2): 108-110.

• 论著·基础研究 • 上一篇    下一篇

旋毛虫Ts87抗原的免疫组化定位研究

杨雅平1, 诸欣平1, 杨静1, 雷丽萍1, 景鹏2   

  1. 1. 首都医科大学基础医学院寄生虫学教研室;2. 首都医科大学解剖学教研室
  • 收稿日期:2002-12-03 修回日期:1900-01-01 出版日期:2003-04-15 发布日期:2003-04-15
  • 通讯作者: 诸欣平

Immunohistochemical Localization of Ts87 Antigen of Trichinella Spiralis

Yang Yaping1, Zhu Xinping1, Yang Jing1, Lei Liping1, Jing Peng2   

  1. 1. Department of Parasitology, Capital. University of Medical Sciences;2. Department of Anatomy, Capital University of Medical Sciences
  • Received:2002-12-03 Revised:1900-01-01 Online:2003-04-15 Published:2003-04-15

摘要: 为了确定旋毛虫Ts87抗原在虫体上的分布及其是否属于旋毛虫排泄分泌(ES)抗原,借助激光扫描共聚焦显微镜,采用免疫组化和Western Bloting等方法进行研究。结果表明:旋毛虫幼虫皮质层富含Ts87蛋白,而且Ts87抗原是旋毛虫排泄分泌抗原中的组分。

关键词: 旋毛虫, 免疫组化定位, 激光扫描共聚焦显微镜

Abstract: In order to know the distribution of Ts87 antigen in Trichinella spiralis larva and to confirm whether Ts87 antigen was one of excretory/secretory antigens of Trichinella spiralis, Western Blotting and immunohistochemical methods observed with the laser scanning confocal microscopy were used. The results shows that Ts87 antigen is one of excretory/secretory antigens of Trichinella spiralis and the cuticular surface of Trichinella spiralis larva is rich in Ts87 protein.

Key words: Trichinella spiralis, immunocytolocalization, laser scanning confocal microscopy

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