应用组织工程方法修复大鼠周围神经损伤的研究

首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (2): 93-96.

应用组织工程方法修复大鼠周围神经损伤的研究

李晓光, 杨朝阳, 张皑峰, 蔡青, 鲁强, 姬曼, 徐群渊, 刘岩

首都医科大学北京神经科学研究所

收稿日期:2003-01-15 修回日期:1900-01-01 出版日期:2003-04-15 发布日期:2003-04-15

Repair of Rats Peripherial Nerve Gap by Tissue Engineering

Li Xiaoguang, Yang Zhaoyang, Zhang Kaifeng, Cai Qing, Lu Qiang, Ji Man, Xu Qunyuan, Liu YanBeijing

Center for Neural Regeneration and Repairing, Capital University of Medical Sciences

Received:2003-01-15 Revised:1900-01-01 Online:2003-04-15 Published:2003-04-15

摘要/Abstract

摘要： 采用雪旺细胞和壳聚糖复合胶原导管,构建组织工程化人工神经,修复大鼠坐骨神经20mm缺损,拟探索一种实验性提高周围神经损伤后修复的质量和速度的方法。术后8周、12周,通过大体观察、组织学染色、神经示踪等技术进行评价。结果表明,术后8周、12周实验组大鼠新生的神经纤维已通过缺损部位,手术局部未出现明显的排斥反应;实验组各项指标优于对照组Ⅱ及对照组Ⅲ,与对照组Ⅰ相近。结果提示:组织工程化人工神经对缺损的坐骨神经修复具有良好的桥梁作用和促进神经生长的作用。

关键词: 人工神经, 壳聚糖, 雪旺细胞, 周围神经再生

Abstract: This study was to explore the possibility of repairing rats sciatic nerve's 20 mm gaps with artificial nerve graft added by Scs. The artificial nerve graft was made of chitosan and collagen. From 8 to 12 weeks after the operation, regenerating nerve joined the nerve ends of the gap. There was no rejection or inflammation. The outcomes of morphometric analysis from artificial nerve graft with Scs were superior to that from control group Ⅱand control group Ⅱ Newly artificial nerve graft with Scs can be a good bridge and promote the nerve to regenerate in reparing the Sciatic nerve gap.

Key words: artificial nerve, chitosan, Schwann cell, peripherial nerve regeneration

中图分类号:

李晓光;杨朝阳;张皑峰;蔡青;鲁强;姬曼;徐群渊;刘岩. 应用组织工程方法修复大鼠周围神经损伤的研究[J]. 首都医科大学学报, 2003, 24(2): 93-96.

Li Xiaoguang;Yang Zhaoyang;Zhang Kaifeng;Cai Qing;Lu Qiang;Ji Man;Xu Qunyuan;Liu YanBeijing. Repair of Rats Peripherial Nerve Gap by Tissue Engineering[J]. Journal of Capital Medical University, 2003, 24(2): 93-96.