聚乙二醇/聚对苯二甲酸丁二醇酯多孔细胞支架的制备与表征

首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (3): 234-236.

聚乙二醇/聚对苯二甲酸丁二醇酯多孔细胞支架的制备与表征

邹静恂¹, 杨华¹, 徐瑞兴¹, 王喆²

1. 首都医科大学基础医学院化学教研室;2. 首都医科大学细胞生物教研室

收稿日期:2003-01-15 修回日期:1900-01-01 出版日期:2003-07-15 发布日期:2003-07-15

Preparation of Porous cell Scaffolds of Poly(ethylene glycal/butylene terephthalate)

Zou Jingxun¹, Yang Hua¹, Xu Ruixing¹, Wang Zhe²

1. Department of Chemistry, School of Basic Sciences, Capital University of Medical Sciences;2. Department of Cellbiology, Capital University of Medical Sciences

Received:2003-01-15 Revised:1900-01-01 Online:2003-07-15 Published:2003-07-15

摘要/Abstract

摘要： 以聚乙二醇/聚对苯二甲酸丁二醇酯(PG/PBT)嵌段共聚物为原料,采用溶液浇铸颗粒滤除法制备了在不同致孔剂粒子尺寸条件下的一系列PEG/PBT多孔细胞支架;用质量法测定其孔隙率;并在PEG/PBT多孔支架上进行细胞培养。研究结果表明:随着制备过程中致孔剂用量的增加,多孔支架的孔隙率逐渐增加,致孔剂的颗粒大小对多孔支架的孔径影响较大,对孔隙率影响较小。在致孔剂用量及致孔剂颗粒尺寸都相同的情况下,随着共聚物中PBT的增加,孔隙率逐渐下降,细胞(培养)在支架上繁殖情况良好,1周后已开始分泌细胞外基质。

关键词: EG/PBT共聚物, 多孔细胞支架, 孔隙率

Abstract: The highly porous biodegradable cell scaffolds of poly(ethylene glycal/butylene terephthalate)were prepared with a solution-casting and porogen particular-leaching technology. A method of a graduated cylinder was used to measure the porosity of the porous scaffold. It was found that the porosity of the porous scaffold increased with the porogen weight fraction, independent of porogen particle sige. As the content of the butylene terephthalate copolymer increased, the porosity of the porous PBT scaffold decreased. The median pore diameter depended on the sige of porogen particle. It was also found that the cartilage proliferated in the PEG/PBTscaffolds and secreted extracellular matrix(ECM).

Key words: poly(ethylene glycal/butylene), porous cell scaffold, porosity

中图分类号:

O622.3

邹静恂;杨华;徐瑞兴;王喆. 聚乙二醇/聚对苯二甲酸丁二醇酯多孔细胞支架的制备与表征[J]. 首都医科大学学报, 2003, 24(3): 234-236.

Zou Jingxun;Yang Hua;Xu Ruixing;Wang Zhe. Preparation of Porous cell Scaffolds of Poly(ethylene glycal/butylene terephthalate)[J]. Journal of Capital Medical University, 2003, 24(3): 234-236.