人胎肝干细胞的分离、培养和鉴定

首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (1): 29-31.

人胎肝干细胞的分离、培养和鉴定

王小平, 陈瑞芬, 宋爱利, 裴雪涛

1. 首都医科大学病理学教研室;2. 军事医学科学院干细胞研究室

收稿日期:2003-02-03 修回日期:1900-01-01 出版日期:2004-01-15 发布日期:2004-01-15

Isolation and Identification of Human Fetal Hepatic Stem Cells

Wang Xiaoping, Chen Ruifen, Song Aili, Pei Xuetao

1. Department of Pathology, Capital University of Medical Sciences;2. Lab of Stem Cell, Academic of Military Medical Sciences

Received:2003-02-03 Revised:1900-01-01 Online:2004-01-15 Published:2004-01-15

摘要/Abstract

摘要： 为探讨人胎肝中干细胞的存在并对其生物学特性进行鉴定,采用原代分离法培养人胎肝细胞集落,免疫组化鉴定细胞集落分子标志物的表达。结果:从人胎肝组织中成功分离表达甲胎蛋白(AFP)、白蛋白、波形蛋白等标志物的胎肝干细胞集落。证实人胎肝中同样存在具有干细胞特性的原始细胞。这一发现为肝干细胞的生物学特性的深入研究奠定了良好的基础.

关键词: 肝干细胞, 集落样生长, 免疫细胞化学

Abstract: The aim was to isolate and identify hepatic stem cells from human fetal liver. H uman fetal l iver cells were isolated and cultured in vitro, the specific markers of cell clo nes were detected immunocytochemically. Then, cell lines were obtained, which was stained positive for specific markers as AFP, albumin, CD₃₄ and CK19. Concl usion could be drawn that Hepatic stem cells lie in human fetal liver which lays a better basis for further research.

Key words: hepatic stem cell, clony, immunoc ytochemistry

中图分类号:

R735.7

王小平;陈瑞芬;宋爱利;裴雪涛. 人胎肝干细胞的分离、培养和鉴定[J]. 首都医科大学学报, 2004, 25(1): 29-31.

Wang Xiaoping;Chen Ruifen;Song Aili;Pei Xuetao. Isolation and Identification of Human Fetal Hepatic Stem Cells[J]. Journal of Capital Medical University, 2004, 25(1): 29-31.

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