反义IGF-Ⅰ寡核苷酸转染对人肝癌细胞增生及凋亡的影响

首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (1): 67-70.

反义IGF-Ⅰ寡核苷酸转染对人肝癌细胞增生及凋亡的影响

乔世峰¹, 孙家邦¹, 朱斌¹, 王季堃²

1. 首都医科大学宣武医院普外科;2. 锦州医学院附属第一医院肿瘤科

收稿日期:2004-01-16 修回日期:1900-01-01 出版日期:2004-01-15 发布日期:2004-01-15

Transfection of Antisense Oligonucleotide Against the Insulin Like Growth Factor I on Proliferation and Apoptosis of Liver Cancer Cell

Qiao Shifeng¹, Sun Jiabang¹, Zhu Bin¹, Wang Jikun²

1. Department of General Surgery, Xuanwu Hospital, Capital University of Medical Sciences;2. Department of Oncology, the First Accessary Hospital of Jinzhou Medical College

Received:2004-01-16 Revised:1900-01-01 Online:2004-01-15 Published:2004-01-15

摘要/Abstract

摘要： 为研究胰岛素样生长因子-I(IGF-I)反义寡核苷酸转染对人肝癌细胞增生、分化及凋亡的影响,探讨寡核苷酸转染治疗肝癌的可行性,利用反义核酸技术,合成针对IGF-I的寡核苷酸片段,利用脂质体包裹反义IGF-I寡核苷酸片段瞬时转染人肝癌细胞系BEL-7402细胞,MTT法检测细胞增生;放免法检测培养细胞上清中AFP、CEA的分泌量;采用末端标记(Tunel)法检测细胞凋亡的变化。结果发现转染反义IGF-I寡核苷酸可使人肝癌细胞系BEL-7402细胞增生下降,AFP、CEA表达降低,凋亡细胞数量增多。反义IGF-I寡核苷酸转染人肝癌细胞系BEL-7402细胞可以降低细胞增生、减少细胞去分化并诱导肝癌细胞凋亡.

关键词: IGF-1, 转染, 反义, 寡核苷酸, 肝癌细胞

Abstract: The study was designed to observe the inhibited effect of the antisense oligonuc leotide(ASON) targeting IGF-I on proliferation,differatiation and apoptosis of the liver cancer cell line BEL-7402 by antisense oligonucleotid e transfection. Antisense oligonucleotide against the insulin like growth factor I was synthesized and transfected to human liver cancer cell line B EL-7402 cells by Lipofectin kits. MTT method was used to measure the cell proli feration. The expression level of CEA and AFP in the supernatant of transfect ce ll culture was measured by RIA. The apoptosis of BEL-7402 cells was observed by TUNEL techniques. It was shown that the lipofectin ASON significantly inhibite d the growth of BEL-7402 cells. The expression of AFP and CEA in the supernatan t of transfected cell culture were significantly lower as compared to the parent cells (P<0.05 and P<0.01 respectively). Antisense IGF-I oligonucle otide transfected to human liver cancer cell line BEL-7402 can lead to a reduct ion of proliferation and differatiation. It can also induce the apoptosis of cells .

Key words: insulin like growth factor I, transfec tion, antisense, oligonucleotides, liver cancer cell

中图分类号:

R73-3
R735.7

乔世峰;孙家邦;朱斌;王季堃. 反义IGF-Ⅰ寡核苷酸转染对人肝癌细胞增生及凋亡的影响[J]. 首都医科大学学报, 2004, 25(1): 67-70.

Qiao Shifeng;Sun Jiabang;Zhu Bin;Wang Jikun. Transfection of Antisense Oligonucleotide Against the Insulin Like Growth Factor I on Proliferation and Apoptosis of Liver Cancer Cell[J]. Journal of Capital Medical University, 2004, 25(1): 67-70.

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