首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (2): 174-176.

• 论著·基础研究 • 上一篇    下一篇

食品红花黄色素抗氧化作用的研究

金鸣, 李金荣, 吴伟   

  1. 北京市心肺血管疾病研究所 首都医科大学附属北京安贞医院药理研究室
  • 收稿日期:2003-10-08 修回日期:1900-01-01 出版日期:2004-04-15 发布日期:2004-04-15

Study on the Antioxidative Effect of Food Safflor Yellow

Jin Ming, Li Jinrong, Wu Wei Beijing   

  1. Institute of Heart, Lung and Blood Vessel Diseases Department of Pharmacology, Beijing Anzhen Hospital, Affiliate of Capital University of Medical Sciences
  • Received:2003-10-08 Revised:1900-01-01 Online:2004-04-15 Published:2004-04-15

摘要: 为观察食品红花黄色素(food safflor yellow,FSY)的抗氧化作用,用邻二氮菲Fe2+氧化法检测FSY清除H2O2/Fe2+体系产生的羟自由基的作用,用硫代巴比妥酸比色法观察小鼠肝匀浆的脂质过氧化,用比色法观察Fe2+/H2O2体系产生的羟自由基致红细胞膜的破裂。发现:2.00~5.33g/LFSY对羟自由基的清除率为29.6%~100%,呈明显的量效关系;0.298~1.194g/LFSY对小鼠肝匀浆脂质过氧化的抑制率为4.6%~70.6%,呈明显的量效关系;0.023~0.228g/LFSY缓解羟自由基引发红细胞破裂的抑制率为16.0%~29.8%。研究结果提示:FSY具有明显的抗氧化作用。

关键词: FSY, 羟自由基, 脂质过氧化, 红细胞

Abstract: The aim was to observe the antioxidative effect of food safflor yellow (FSY). Hydroxyl radical scavenge effect of FSY was tested with 1,10-phenanthroline-Fe2+ oxidative assay. Lipid peroxidation of mouse liver suspension was measured with thiobarbituric acid colorimetry technique. Hemocytocatheresis was determined with colorimetry. Hydroxyl radical can be scavenged by 2.00 to 5.33 g/L FSY dose dependently. Mouse liver suspension peroxidation was inhibited by 0.298 to 1.194 g/L FSY dosage dependently. Hemocytocatheresis was attenuated by 0.023 to 0.228 g/L FSY dose dependently. FSY is effective against oxidative damage.

Key words: food safflor yellow, hydroxyl radical, lipid peroxidation, hemocytocatheresis

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