儿茶提取物对流感病毒感染小鼠免疫功能的影响

首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (2): 180-182.

儿茶提取物对流感病毒感染小鼠免疫功能的影响

郑群, 平国玲, 赵文明

首都医科大学微生物学教研室

收稿日期:2003-07-16 修回日期:1900-01-01 出版日期:2004-04-15 发布日期:2004-04-15

Immunomodulatory Effect of Catechu Extract on Infectious Animal Model

Zheng Qun, Ping Guoling, Zhao Wenming

Department of Microbiology, Capital University of Medical Sciences

Received:2003-07-16 Revised:1900-01-01 Online:2004-04-15 Published:2004-04-15

摘要/Abstract

摘要： 用流感病毒鼠肺适应株A/FM/1/47(H1N1)感染的小鼠肺炎为模型,研究儿茶提取物对流感病毒感染机体免疫功能的影响,包括对迟发型超敏反应(DTH)及中和抗体产生的影响。结果显示:儿茶提取物可显著增强2,4-二硝基氟苯(DNFB)诱发的小鼠DTH反应,即儿茶素+病毒感染组和儿茶素对照组小鼠的耳肿胀度和脾指数都明显增加,与正常对照组相比有显著性差异(P<0.05);提示:儿茶提取物具有增强小鼠T细胞免疫功能的作用。儿茶提取物实验组的中和抗体平均效价为1:203,与病毒感染对照组(平均效价为1:104.2)相比具有非常显著性差异(P<0.01);说明儿茶提取物可增强流感病毒感染小鼠中和抗体的水平,提示其对小鼠体液免疫功能亦有增强作用。研究表明:儿茶提取物具有增强病毒感染机体的细胞免疫和体液免疫功能的作用。

关键词: 儿茶提取物, 流感病毒, DTH反应, 中和抗体

Abstract: The objective was to investigate the immunomodulatory effect of catechu extract with the infectious animal model, which were measured by delayed type hypersensitivity (DTH) and the neutralizing antibody level. The animal model of virus infection of respiratory tract was established by primed influenza virus strain A/FM/1/47(H1N1). The results showed the catechu extract could markedly intensify DTH caused by DNFB. It could also increase ear swelling and spleen index in the group of catechu extract and virus infection and the group only treated by catechu extract, compared with the control group (P<0.05). The average titer of neutralizing antibody of the group treated by catechu extract was 1:203. It had significantly increased compared with the control group without catechu extract (1:104.2)(P<0.01). The finding suggests that the catechu extract could markedly intensify the cell immunological function and the humoral immunological function in mice infected by influenza virus.

Key words: catechu extract, influenza virus, delayed type hypersensitivity, neutralizing antibody

中图分类号:

R373.1⁺3

郑群;平国玲;赵文明. 儿茶提取物对流感病毒感染小鼠免疫功能的影响[J]. 首都医科大学学报, 2004, 25(2): 180-182.

Zheng Qun;Ping Guoling;Zhao Wenming. Immunomodulatory Effect of Catechu Extract on Infectious Animal Model[J]. Journal of Capital Medical University, 2004, 25(2): 180-182.

[1]	李刚. 甲型H1N1流感病毒的分子特征[J]. 首都医科大学学报, 2009, 30(3): 267-270.
[2]	赵文明;郑群;刘振龙;平国玲;丁丽新;石伟先;刘海林. 儿茶提取物抗甲型流感病毒作用的实验研究[J]. 首都医科大学学报, 2005, 26(2): 167-170.
[3]	郑群;平国玲;赵文明. 儿茶提取物抗流感病毒作用的小鼠体内实验研究[J]. 首都医科大学学报, 2004, 25(1): 32-34.