蚯蚓组织成分对成纤维细胞增生作用的实验研究

首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (3): 317-320.

蚯蚓组织成分对成纤维细胞增生作用的实验研究

崔泓¹, 于培兰², 孙林², 张祖珣², 张海燕², 李卫红²

1. 首都医科大学中国康复研究中心;2. 首都医科大学基础医学院

收稿日期:2003-08-27 修回日期:1900-01-01 出版日期:2004-07-15 发布日期:2004-07-15

Mitogenic Activity of Fibroblast in Earthworm

Cui Hong¹, Yu Peilan², Sun Lin², Zhang Zuxun², Zhang Haiyan², Li Weihong²

1. Rehabilitation Research Center, Capital University of Medical Sciences;2. Master Candidate of Equal Education, Capital University of Medical Sciences

Received:2003-08-27 Revised:1900-01-01 Online:2004-07-15 Published:2004-07-15

摘要/Abstract

摘要： 通过蚯蚓组织成分对成纤维细胞增生作用的实验研究,探讨其对创伤愈合的作用机制.用醋酸盐缓冲液抽提蚯蚓组织成分,经硫酸铵分段盐析、阴离子交换层析、凝胶过滤层析等方法,分离提取到1组蛋白质成分.采用MTT法分析各分离组分对小鼠成纤维细胞增生作用的影响.结果:经分离提取促增生组分位于SephadexG100层析第1峰,经SDS-聚丙烯酰胺凝胶电泳显示此促增生组分为3条区带,相对分子质量范围57000、72000、87000.经实验证明蚯蚓组织中含有能够促进成纤维细胞增生的活性成分.

关键词: 蚯蚓, 成纤维细胞, 创伤愈合

Abstract: By means of experiment, the affect of the tissue components purified from earthworm on the mitogenic activity of fibroblast was studied to investigate its pharmic mechanism in the wound healing.In the experiments, using acetate buffer the tissues components were purified from the earthworm, which was crude extract.A group of proteins were obtained from the crude extract of the earthworm through ammonium sulfate precipitation, ion exchange chromatography and gel filtration chromatography.The effect of the group of proteins on the mitogenic activity of fibroblast was investigated with MTT method.It was illustrated that the group of proteins of mitogenic activity was on the first peak of Sephadex-G100, and presented three bands in SDS polyacrylamide gel electrophoresis.The range of their molecular weighs were 57 000, 72 000, 87 000.It can be drawn that there are some kind of proteins in the tissue components of the earthworm that has mitogenic activity of fibroblast.

Key words: earthworm, fibroblast, wound healing

中图分类号:

R282.74
R641

崔泓;于培兰;孙林;张祖珣;张海燕;李卫红. 蚯蚓组织成分对成纤维细胞增生作用的实验研究[J]. 首都医科大学学报, 2004, 25(3): 317-320.

Cui Hong;Yu Peilan;Sun Lin;Zhang Zuxun;Zhang Haiyan;Li Weihong. Mitogenic Activity of Fibroblast in Earthworm[J]. Journal of Capital Medical University, 2004, 25(3): 317-320.

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