李氏5号方对Aβ导致神经元毒性的保护作用

首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (4): 472-474.

李氏5号方对Aβ导致神经元毒性的保护作用

张景艳¹, 王蓉¹, 李子中², 姬志娟¹, 程浩然², 李生海², 盛树力¹

1. 首都医科大学宣武医院北京脑老化重点实验室;2. 广东省高明脑病医疗医药研究院

收稿日期:2003-10-24 修回日期:1900-01-01 出版日期:2004-10-15 发布日期:2004-10-15

Lishi No.5 Formula Capsule on Neurotoxicity Induced by Aβ

Zhang Jingyan¹, Wang Rong¹, Li Zizhong², Ji Zhijuan¹, Cheng Haoran², Li Shenghai², Sheng Shuli¹

1. Beijing Research Laboratory for Brain Aging, Xuanwu Hospital, Capital University of Medical Sciences;2. Guangdong Gao Ming Encephalopathy Medical and Pharmacological Academy

Received:2003-10-24 Revised:1900-01-01 Online:2004-10-15 Published:2004-10-15

摘要/Abstract

摘要： 观察李氏5号方对人神经母细胞瘤株SY5Y的细胞生长情况和对β-淀粉样肽(β-amyloid,Aβ)导致神经毒性的影响,从而在细胞水平上证实该中药对神经细胞的保护作用.用固相法合成Aβ_25-35,高效液相纯化,李氏5号方水提液0.5 g/mL.将人神经母细胞瘤株SY5Y细胞接种后分为正常对照组、Aβ_25-35 25 μmol/L损伤组和Aβ_25-35 25 μmol/L加李氏5号方水提液保护组.以噻唑蓝(MTT)代谢率、乳酸脱氢酶(LDH)漏出率、细胞轴突长度、胞体面积为观察指标.结果:与正常对照组相比,Aβ_25-35组SY5Y细胞的轴突长度和胞体面积均较小,MTT代谢率降低,LDH漏出率升高,而加入李氏5号方保护后,可使上述指标恢复或接近正常.提示:李氏5号方具有神经保护作用,可减轻Aβ导致的神经毒性.

关键词: 李氏5号方, Aβ_25-35, 神经营养作用, 人神经母细胞瘤株SY5Y

Abstract: The aim was to observe the the effect of Lishi No.5 Formula capsule on the growth of human neuroblastoma cell line SY5Y and on the neurotoxicity induced by Aβ. Aβ_25-35 was synthesized by solid phase method and purified by HPLC. Lishi No.5 Formula water extract was 0.5 g/mL. Human neuroblastoma cells SY5Y were segregated into 3 groups: normal control group; Aβ_25-35(25 μmol/L) damaged group; and Aβ_25-35(25 μmol/L) + Lishi No.5 Formula water extract (corresponding to Lishi No.5 Formula 0.5 g/L culture medium) for neuroprotection group. MTT metabolic rate, LDH leakage rate, axonal length, area of cell body were used as indicators. Compare with normal control group, Aβ_25-35 damaged group showed reduced MTT metabolic rate, increased LDH leakage rate, and diminished axonal length and area of cell body, but the addition of Lishi No.5 Formula normalized the foregoing changes. Lishi No.5 Formula has neuroprotective effect. It can diminish neurotoxicity of Aβ.

Key words: Lishi No.5 Formula, Aβ_25-35, neurotrophic effect, human neuroblastoma cell line SY5Y

中图分类号:

R285.6

张景艳;王蓉;李子中;姬志娟;程浩然;李生海;盛树力. 李氏5号方对Aβ导致神经元毒性的保护作用[J]. 首都医科大学学报, 2004, 25(4): 472-474.

Zhang Jingyan;Wang Rong;Li Zizhong;Ji Zhijuan;Cheng Haoran;Li Shenghai;Sheng Shuli. Lishi No.5 Formula Capsule on Neurotoxicity Induced by Aβ[J]. Journal of Capital Medical University, 2004, 25(4): 472-474.

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[2]	王蓉;张景艳;姬志娟;徐艳玲;盛树力. APP17肽对Aβ导致神经元毒性作用的影响[J]. 首都医科大学学报, 2001, 22(3): 202-205.