儿茶提取物抗甲型流感病毒作用的实验研究

首都医科大学学报 ›› 2005, Vol. 26 ›› Issue (2): 167-170.

儿茶提取物抗甲型流感病毒作用的实验研究

赵文明¹, 郑群¹, 刘振龙¹, 平国玲¹, 丁丽新², 石伟先², 刘海林²

1. 首都医科大学微生物学教研室;2. 北京市疾病预防控制中心

收稿日期:2004-03-22 修回日期:1900-01-01 出版日期:2005-04-24 发布日期:2005-04-24
通讯作者: 郑群

Effect of Chinese Herb-catechu Extract on Influenza A Virus

Zhao Wenming¹, Zheng Qun¹, Liu Zhenlong¹, Ping Guoling¹, Ding Lixin², Shi Weixian², Liu Hailin²

1. Department of Microbiology, Capital University of Medical Sciences;2. Ding Lixin, Shi Weixian, Liu Hailin Beijing Centers for Disease Control and Prevention

Received:2004-03-22 Revised:1900-01-01 Online:2005-04-24 Published:2005-04-24

摘要/Abstract

摘要：

目的研究从中药儿茶中提取的药物成分抗甲型流感病毒的作用。方法用狗肾传代MDCK细胞培养法观察儿茶提取物抑制甲型流感病毒的致细胞病变作用;用鸡胚培养法观察儿茶提取物抑制甲型流感病毒增生的作用;用红细胞凝集试验观察儿茶提取物体外直接抑制甲型流感病毒的作用。结果 1)儿茶提取物的细胞毒性较低,可有效抑制甲型流感病毒感染细胞(P<0.05);2)儿茶提取物在一定范围内可明显抑制甲型流感病毒在鸡胚内的增生,经红细胞凝集试验测定抑制病毒效价为8倍或8倍以上;3)儿茶提取物直接与甲型流感病毒作用后可抑制病毒血凝效价达16倍以上。结论儿茶提取物具有很好的抗甲型流感病毒的作用。

关键词: 儿茶提取物, 甲型流感病毒, MDCK细胞培养法, 鸡胚培养法, 红细胞凝集试验

Abstract:

Objective To investigate the inhibitory effect of catechu extract to influenza Avirus. Methods The inhibitory cytopathic effects (CPE) of catechu extract on influenza Avirus were observed by MDCK cell culture techniques. The inhibiting influenza Avirus effect of catechu extract was observed by means of chick-embryo culture, and the inhibitory activity of catechu extract on influenza Avirus in vitro was observed by the hemagglutination inhibition test. Results 1) Cytotoxicity of catechu extract was lower and the catechu extract could inhibit influenza Avirus from infecting MDCK significantly. 2) The catechu extract showed significant inhibition on the influenza Avirus in the groups within the limits of dose on chick-embryo culture and the hemagglutination inhibition titer was over 8 times higher than the control group. 3) The catechu extract had distinctly inhibitory functions on influenza Avirus, and the hemagglutination inhibition titer was over 16 times higher than the control group. Conclusion The chinese herb-catechu extract has strong effect on anti-influenza Avirus.

Key words: catechu extract, influenza A virus, MDCK cell culture techniques, chick-embryo culture, hemagglutination test

中图分类号:

R373

赵文明;郑群;刘振龙;平国玲;丁丽新;石伟先;刘海林. 儿茶提取物抗甲型流感病毒作用的实验研究[J]. 首都医科大学学报, 2005, 26(2): 167-170.

Zhao Wenming;Zheng Qun;Liu Zhenlong;Ping Guoling;Ding Lixin;Shi Weixian;Liu Hailin. Effect of Chinese Herb-catechu Extract on Influenza A Virus[J]. Journal of Capital Medical University, 2005, 26(2): 167-170.