北京地区呼吸道合胞病毒分离株F蛋白基因序列分析

首都医科大学学报 ›› 2005, Vol. 26 ›› Issue (5): 565-567.

北京地区呼吸道合胞病毒分离株F蛋白基因序列分析

芦起¹, 申昆玲¹, 张燕², 刘亚谊¹, 赵宇红¹, 刘春艳¹, 姬奕新², 谢正德¹, 许文波²

1. 首都医科大学附属北京儿童医院病毒室;2. 中国疾病预防控制中心病毒疾病预防控制所国家麻疹室

收稿日期:2005-08-20 修回日期:1900-01-01 出版日期:2005-10-24 发布日期:2005-10-24

Sequence Analysis of F Protein Gene of Respiratory Syncytial Virus in Beijing Area

Lu Qi¹, Shen Kunling¹, Zhang Yan², Liu Yayi¹, Zhao Yuhong¹, Liu Chunyan¹, Ji Yixin², Xie Zhengde¹, Xu Wenbo²

1. Laboratory for Virus, Beijing Children's Hospital, Capital University of Medical Sciences;2. National Laboratory for Measles, National Institute for Viral Disease Control and Prevention, China CDC

Received:2005-08-20 Revised:1900-01-01 Online:2005-10-24 Published:2005-10-24

摘要/Abstract

摘要：

目的分析中国北京地区4株呼吸道合胞病毒(RSV)F蛋白的基因序列并与RSV原型株A₂进行比较,得出中国北京地区RSV F蛋白基因是否存在变异及变异特征。方法用反转录聚合酶链反应(RT-PCR)分2段扩增F蛋白的基因核苷酸序列,将得到的P₁、P₂2段切胶纯化标记后测序,应用Bioedit软件对得到的序列做碱基配对和比较分析。结果 4株RSV F蛋白核苷酸序列的同源性为94.92%～95.62%。信号肽区的同源性为99.36%～99.73%。F1、F2亚单位同源性分别为98.93%～99.31%和96.69%～97.33%。氨基酸同源性分别为:F蛋白的蛋白编码区97.39%～97.74%,信号肽区99.13%～99.65%,F1、F2亚单位98.78%～99.48%、98.61%～99.30%。结论中国RSV北京株与原型株A₂之间的F蛋白基因变异小,具有很高的同源性。氨基酸在抗原位点Ⅱ保守,此段氨基酸可作为RSV疫苗的候选位点。

关键词: 呼吸道合胞病毒, F蛋白, 核苷酸序列

Abstract:

Objective To study the sequence of F protein gene of 4 respiratory syncytial virus strains in Beijing area of China.Methods P₁ and P₂ segments of respiratory syncytial viruses were amplified by RT-PCR,then the PCR products were purified for sequencing.Analysis was carried out by Bioedit program.Results The nucleotide sequences of the four RSV strains F mRNA had 94.92%～95.62% overall nucleotide identity with those of RSV isolate A₂,the nucleotide sequences of signal peptide of four RSV strains F mRNA had 99.36%～99.73% identity,the F1 chain had 98.93%～99.31%,F2 chain had 96.69%～97.33% identity respectively.The deduced aminoacid sequences of the four strains and A₂ F proteins had 97.39%～97.74% overall aminoacid identity,the signal peptide had 99.13%～99.65%,the F1 chain had 98.78%～99.48%,F2 chain had 98.61%～99.30% identity respectively.Conclusion The differences between the A₂ and Beijing strains in the F gene are small,so the nucleotide and aminoacid have high identity.The antigenic area Ⅱ could be the potential site of RSV vaccine.

Key words: respiratory syncytial virus, F protein, nucleotide sequence

中图分类号:

R725.6

芦起;申昆玲;张燕;刘亚谊;赵宇红;刘春艳;姬奕新;谢正德;许文波. 北京地区呼吸道合胞病毒分离株F蛋白基因序列分析[J]. 首都医科大学学报, 2005, 26(5): 565-567.

Lu Qi;Shen Kunling;Zhang Yan;Liu Yayi;Zhao Yuhong;Liu Chunyan;Ji Yixin;Xie Zhengde;Xu Wenbo. Sequence Analysis of F Protein Gene of Respiratory Syncytial Virus in Beijing Area[J]. Journal of Capital Medical University, 2005, 26(5): 565-567.

[1]	张利群;陈杭薇;王四海;辛庆红;杜玉国;尤兰华. 人呼吸道合胞病毒转录调节基因转染PAa和A549肺腺癌细胞系[J]. 首都医科大学学报, 2005, 26(6): 733-736.
[2]	赵宇红;申昆玲;刘亚谊;刘春艳;谢正德. 鱼腥草注射液治疗呼吸道合胞病毒感染的体外实验研究[J]. 首都医科大学学报, 2005, 26(5): 571-573.
[3]	卢令格;驹田雅之;喜多村直实. 人Hrs cDNA的克隆与序列分析[J]. 首都医科大学学报, 1997, 18(3): 238-241.