血管组织工程生物材料细胞相容性的评价

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (3): 321-324.

血管组织工程生物材料细胞相容性的评价

卢光, 张建, 李建新, 谷涌泉, 孙海晨, 陈亮, 刘庆阳, 汪忠镐

首都医科大学宣武医院血管外科,首都医科大学血管外科研究所

收稿日期:2005-10-29 修回日期:1900-01-01 出版日期:2006-06-24 发布日期:2006-06-24

Evaluation of Cytocompatibility of Several Biomaterials on Vascular Tissue Engineering

Lu Guang, Zhang Jian, Li Jianxin, Gu Yongquan, Sun Haichen, Chen Liang, Liu Qingyang, Wang Zhonggao

Department of Vascular Surgery,Xuanwu Hospital, Capital University of Medical Sciences; The Institute of Vascular Surgical Research, Capital University of Medical Sciences

Received:2005-10-29 Revised:1900-01-01 Online:2006-06-24 Published:2006-06-24

摘要/Abstract

摘要：

目的探讨以聚羟基丁酸戊酸酯(PHBV)为主体的几种生物材料和人包皮成纤维细胞的生物相容性,为组织工程血管初步选择较好的支架材料.方法将以PHBV为主体的几种生物材料和人包皮成纤维细胞体外培养,并对材料的接触角、细胞的形态和细胞的增生情况进行测定.结果所有的材料中,PHBV生物材料接触角最小,与细胞的相容性最好.结论 PHBV具有良好的细胞相容性,从而为组织工程血管初步筛选出一种较好的生物材料.

关键词: 生物材料, 细胞相容性, 组织工程血管, 荧光染色

Abstract:

Objective To study the cytocompatibility of several biomaterials such as polyhydroxybutyrate-polyhydroxyvalerate(PHBV) with human fibroblast cell and provide the better materials for vascular tissue engineering.Methods The fibroblast cell were cultured on the surface of several biomaterials in vitro, then the morphological characters were observed with fluorescence microscope and cell proliferation were detected with MTT assay. In addition, the biomaterials contact angle were measured.Results In all biomaterials, PHBV's contact angle was the smallest and it's cytocompatibility is the best.Conclusion PHBV has good cytocompatibility and could be the better biomaterial for the vascular tissue engineering.

Key words: biomaterials, cytocompatibility, tissue engineering blood vessel, fluorescence staining

中图分类号:

R318.08

卢光;张建;李建新;谷涌泉;孙海晨;陈亮;刘庆阳;汪忠镐. 血管组织工程生物材料细胞相容性的评价[J]. 首都医科大学学报, 2006, 27(3): 321-324.

Lu Guang;Zhang Jian;Li Jianxin;Gu Yongquan;Sun Haichen;Chen Liang;Liu Qingyang;Wang Zhonggao . Evaluation of Cytocompatibility of Several Biomaterials on Vascular Tissue Engineering[J]. Journal of Capital Medical University, 2006, 27(3): 321-324.

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[2]	历俊华;万虹;翟晶;王忠诚. 无血清饥饿法去除神经组织培养中的成纤维细胞[J]. 首都医科大学学报, 2004, 25(2): 205-207.