大蒜素对肺癌A549细胞株β防御素表达的影响

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (6): 792-794.

大蒜素对肺癌A549细胞株β防御素表达的影响

司利钢¹, 刘玺诚¹, 李文梅², 吕有勇², 饶小春¹, 马渝燕¹

1. 首都医科大学附属北京儿童医院儿科研究所;2. 北京大学临床肿瘤学院北京市肿瘤防治所

收稿日期:2006-04-20 修回日期:1900-01-01 出版日期:2006-12-24 发布日期:2006-12-24

The Different Expression Levels of Human Beta Defensins in the Lung Cancer Cell Line A549 Cells after Treated with Allicin

Si Ligang¹, Liu Xicheng¹, Li Wenmei², Lv Youyong², Rao Xiaochun¹, Ma Yuyan¹

1. Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital University of Medical Sciences;2. Molecular Biology Laboratory, Beijing Institute for Cancer Research, School of Oncology, Peking University

Received:2006-04-20 Revised:1900-01-01 Online:2006-12-24 Published:2006-12-24

摘要/Abstract

摘要： 目的 β防御素(human beta defensins,HBD)是人体产生的天然抗菌物质,本研究通过大蒜素对β防御素表达的影响,探讨大蒜素的免疫调节机制.方法采用含大蒜素的细胞培养液处理人肺癌A549细胞,观察其对A549细胞不同种类β防御素mRNA表达水平的影响.结果含5 μg/mL的大蒜素细胞培养液可使HBD3 mRNA的表达上调,而对HBD2和HBD1的表达无显著影响.结论大蒜素可以使A549细胞HBD3的表达升高,这可能是大蒜素参与人体免疫调节,提高机体免疫力的机制之一.大蒜素处理后HBD3升高而HBD2水平,不变进一步证实了HBD3与HBD2的诱导表达途径不同.

关键词: β防御素, 大蒜素, 抗菌肽, 诱导表达

Abstract: Objective Human beta defensins(HBD) are antimicrobial peptides mainly generated by the epithelia of human body,Allicin is a antimicrobial drug made in China,our research is to investigate what might be the mechanism involved in improving the immunity of human body. Methods We used RPMI 1640 which included 5 μg/mL Allicin to culture the lung cancer cell line A549,and detected the mRNA expression levels of different kinds of HBD(HBD1～3). Results The HBD3 expression level could be improved,and there were no effects on the expression level of HBD1 and HBD2. Conclusion Allicin can improve the expression level of HBD3 in the cell line A549,that might be one of the reasons which allicin involve in the adjustment of human immunity.It further confirmed that there were different signalling pathways between HBD3 and HBD2.

Key words: human beta defensins, allicin, antimicrobial peptides, induced expression

中图分类号:

Q786

司利钢;刘玺诚;李文梅;吕有勇;饶小春;马渝燕. 大蒜素对肺癌A549细胞株β防御素表达的影响[J]. 首都医科大学学报, 2006, 27(6): 792-794.

Si Ligang;Liu Xicheng;Li Wenmei;Lv Youyong;Rao Xiaochun;Ma Yuyan. The Different Expression Levels of Human Beta Defensins in the Lung Cancer Cell Line A549 Cells after Treated with Allicin[J]. Journal of Capital Medical University, 2006, 27(6): 792-794.

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