常见革兰阳性细菌鉴定与耐药检测基因芯片的临床应用

首都医科大学学报 ›› 2007, Vol. 28 ›› Issue (2): 140-144.

常见革兰阳性细菌鉴定与耐药检测基因芯片的临床应用

王雅杰¹, 王璨^2,3, 方芳¹, 史从宁¹, 蒋迪², 张国军¹, 吕虹¹, 杨华卫², 常铮¹

1. 首都医科大学附属北京天坛医院实验诊断中心;2. 博奥生物有限公司暨生物芯片北京国家工程研究中心;3. 清华大学医学院

收稿日期:2007-01-17 修回日期:1900-01-01 出版日期:2007-04-24 发布日期:2007-04-24
通讯作者: 康熙雄;高华方

The Clinical Application of DNA Chip for Common Gram-positive Bacteria Identification and Drug Resistance Detection

Wang Yajie¹, Wang Can^2,3, Fang Fang¹, Shi Congning¹, Jiang Di², Zhang Guojun¹, Lü Hong¹, Yang Huawei², Chang Zheng¹

1. Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University;2. Capital Bio Corporation NationalEngineering Research Center for Beijing Biochip Technology;3. School of Medicine, Tsinghua University

Received:2007-01-17 Revised:1900-01-01 Online:2007-04-24 Published:2007-04-24

摘要/Abstract

摘要：

目的利用基因芯片技术进行常见革兰阳性细菌的细菌鉴定和耐药性检测。方法用生理生化鉴定和药敏纸片法确定445份革兰阳性细菌菌株的细菌种类和耐药性,盲法进行革兰阳性细菌鉴定与耐药检测基因芯片的检测,用SPSS 10.0 for Windows软件统计分析。结果与临床常规方法相比,耐药检测基因芯片鉴定指标的灵敏度均>96%,特异度均>98%;耐药检测指标的灵敏度均>94%,特异度均>90%。χ²检验结果P值均>0.05,基因芯片检测结果与临床常用方法检测结果差异无统计学意义。Kappa值均>0.75,2种方法一致性较好。结论基因芯片技术对细菌鉴定和耐药检测有一定的临床应用价值。

关键词: 细菌鉴定, 耐药, 基因芯片

Abstract:

Objective To study the clinical application of DNA chip for common Gram-positive bacteria identification and drug resistance detection. Methods 445 Gram-positive strains were identified with physiological and biochemical methods, and were detected of drug resistance with diffusion disk method for susceptibility test. Then the strains were blindly detected with using DNA chip for identification and drug resistance. The data were analyzed using SPSS 10.0 for Windows software. Results The sensitivity of all identification indexes of DNA chip were higher than 96%, and all of their specificity were higher than 98%. The sensitivity of all drug resistance indexes of DNA chip were higher than 94%, and all of their specificity were higher than 90%. The chi square test values of all indexes were higher than 0.05, showing that there was no significant difference between DNA chip and the clinical routine methods. The Kappa values of all indexes were higher than 0.75, indicating that the coincidence between the two kinds of methods was good. Conclusion There is clinical potential of gene chip technology for bacteria identification and drug resistance detection.

Key words: bacteria identification, drug resistance, gene chip

中图分类号:

R446.5

王雅杰;王璨;方芳;史从宁;蒋迪;张国军;吕虹;杨华卫;常铮. 常见革兰阳性细菌鉴定与耐药检测基因芯片的临床应用[J]. 首都医科大学学报, 2007, 28(2): 140-144.

Wang Yajie;Wang Can;Fang Fang;Shi Congning;Jiang Di;Zhang Guojun;Lü Hong;Yang Huawei;Chang Zheng. The Clinical Application of DNA Chip for Common Gram-positive Bacteria Identification and Drug Resistance Detection[J]. Journal of Capital Medical University, 2007, 28(2): 140-144.

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