健脾补肾方对小鼠记忆障碍模型的影响

首都医科大学学报 ›› 2009, Vol. 30 ›› Issue (1): 24-26.

健脾补肾方对小鼠记忆障碍模型的影响

贾竑晓¹, 何淑嫦², 唐永怡¹, 江镇康¹, 张继志¹

1. 首都医科大学附属北京安定医院;2. 北京大学心理学系

收稿日期:2008-11-18 修回日期:1900-01-01 出版日期:2009-02-21 发布日期:2009-02-21
通讯作者: 唐永怡

Effect of Jianpibushenfang on Ketamine-induced Memory Impairment in Mice

Jia Hongxiao¹, He Shuchang², Tang Yongyi¹, Jiang Zhenkang¹, Zhang Jizhi¹

1. Beijing Anding Hospital, Capital Medical University;2. Department of Psychological, Peking University

Received:2008-11-18 Revised:1900-01-01 Online:2009-02-21 Published:2009-02-21

摘要/Abstract

摘要： 目的研究健脾补肾方(Jian Pi Bu Shen Fang,JPBSF)对氯胺酮所致小鼠记忆障碍的影响。方法用跳台法测定小鼠训练时和24 h后重测时的错误次数。分别于训练前20 min腹腔注射氯胺酮和训练前10 min腹腔注射氢溴酸樟柳碱,复制2种小鼠记忆获得障碍模型。用氨基酸自动分析仪测定小鼠脑内谷氨酸的含量。结果氯胺酮模型组小鼠学习记忆实验重测后错误次数明显多于对照组(P<0.001),JPBSF小剂量+氯胺酮组、JPBSF大剂量+氯胺酮组和吡拉西坦+氯胺酮组小鼠学习记忆实验重测后错误次数明显少于氯胺酮模型组(P<0.01),氯胺酮模型组和JPBSF小剂量+氯胺酮组小鼠学习记忆实验重测后脑内谷氨酸含量差异无统计学意义(P>0.05);樟柳碱模型组小鼠学习记忆实验重测后错误次数明显多于对照组(P<0.001),JPBSF小剂量+樟柳碱组、JPBSF大剂量+樟柳碱组和吡拉西坦+樟柳碱组小鼠学习记忆实验重测后错误次数明显少于樟柳碱模型组(P<0.01)。结论 JPBSF对氯胺酮造成小鼠记忆获得障碍有明显的改善作用,但不增加小鼠脑内谷氨酸含量,JPBSF对樟柳碱造成小鼠记忆获得障碍亦有明显改善作用。

关键词: 健脾补肾方, 记忆障碍, 氯胺酮, 谷氨酸

Abstract: Objective To investigate the effect of Jianpibushenfang(JPBSF) on Ketamine-induced memory impairment in mice. Methods Memory errors of mice in training and retesting 24 hours later were measured by Step-down method. The memory acquisition impairments were induced by i.p Ketamine 20 and Anisodine 10 minutes before training in Step-down performance, respectively. Glutamate in mice brain was analyzed by automated amino acid assay. Results Memory errors of saline+ketamine group in retesting was more significant than that of saline+saline group(P<0.001), while memory errors of JPBSF low dose+ketamine group, JPBSF large dose+ketamine group and piracetam+ketamine group were significantly milder than that of saline+ketamine group(P<0.01). There was no difference in glutamate content in mice brain between JPBSF low dose+ketamine group and saline+ketamine group(P>0.05). Memory errors of saline+anisodine group in retesting was obviously more than that of saline+saline group(P<0.001), while memory errors of JPBSF low dose+anisodine group, JPBSF large dose+anisodine group and piracetam+ketamine group were significantly milder than that of saline+anisodine group(P<0.01). Conclusion JPBSF has improved ketamine and anisodine-induced memory acquisition impairment, but did not change the glutamate level in mice brain.

Key words: Jianpibushenfang, memory impairment, ketamine, glutamate

中图分类号:

贾竑晓;何淑嫦;唐永怡;江镇康;张继志. 健脾补肾方对小鼠记忆障碍模型的影响[J]. 首都医科大学学报, 2009, 30(1): 24-26.

Jia Hongxiao;He Shuchang;Tang Yongyi;Jiang Zhenkang;Zhang Jizhi. Effect of Jianpibushenfang on Ketamine-induced Memory Impairment in Mice[J]. Journal of Capital Medical University, 2009, 30(1): 24-26.

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[2]	孙艳霞;盛开花;李天佐;纪方;张炳熙. 氯胺酮与利多卡因在儿童麻醉诱导中应用的可行性研究[J]. 首都医科大学学报, 2005, 26(2): 207-210.
[3]	刘冬梅;杨荪;周以健;王蓬文;王兴翠. 谷氨酸单钠对大鼠睾丸的影响[J]. 首都医科大学学报, 1998, 19(4): 319-322.
[4]	刘冬梅;杨荪;王蓬文;周以健. 谷氨酸单钠对大鼠垂体促性腺激素细胞的影响[J]. 首都医科大学学报, 1998, 19(3): 213-216.
[5]	鲁晓莉;高清玲;丁育基;陈文东;李雅莉;李林. 氯化钾对神经母细胞瘤细胞系谷氨酸释放的影响[J]. 首都医科大学学报, 1998, 19(3): 246-249.
[6]	王秀琴;曾晓蓓;米娜娃;杨荪;王蓬文. 谷氨酸单钠致大鼠肾上腺组织化学变化的研究[J]. 首都医科大学学报, 1998, 19(2): 109-112.
[7]	王蓬文;杨佩荪;刘国贞;汪红焱. 谷氨酸单钠对大鼠垂体生长激素细胞发育的影响[J]. 首都医科大学学报, 1997, 18(3): 214-217.
[8]	李玉兰;李郁英;安云庆;杨佩荪;刘冬梅. 谷氨酸单钠对大鼠发育和免疫功能的影响[J]. 首都医科大学学报, 1997, 18(3): 218-221.
[9]	鲁厚祯;王卫;郎晓燕;杨连雪;杨佩荪;王蓬文. 谷氨酸钠致使新生大鼠海马锥体细胞损伤的形态学研究[J]. 首都医科大学学报, 1996, 17(3): 168-172.
[10]	杨珮荪;林锴;王蓬文;刘国贞. 谷氨酸单钠对大鼠骨发育的影响[J]. 首都医科大学学报, 1994, 15(3): 184-187.
[11]	杨珮荪;林锴;盛树力;高林. 谷氨酸单钠引起大鼠垂体性侏儒变化[J]. 首都医科大学学报, 1990, 11(4): 283-288.