北京市部分男男同性恋人群中HIV-1分子流行病学研究

doi:10.3969/j.issn.1006-7795.2009.05.010

首都医科大学学报 ›› 2009, Vol. 30 ›› Issue (5): 616-620.doi: 10.3969/j.issn.1006-7795.2009.05.010

• 艾滋病基础研究及治疗 • 上一篇下一篇

北京市部分男男同性恋人群中HIV-1分子流行病学研究

刘志英¹, 李海英², 张彤², 黄晓婕², 魏飞力¹, 徐萌¹, 焦艳梅¹, 徐小宁², 陈德喜¹, 吴昊²

1. 首都医科大学附属北京佑安医院性病艾滋病实验室;2. 首都医科大学附属北京佑安医院感染科

收稿日期:2009-06-16 修回日期:1900-01-01 出版日期:2009-10-21 发布日期:2009-10-21
通讯作者: 吴昊

Molecular Epidemiologic Study on Human Immunodeficiency Virus Type 1 Infection in Partial Men Who have Sex with Men(MSM) in Beijing

LIU Zhi-ying¹, LI Hai-ying², ZHANG Tong², HUANG Xiao-jie², WEI Fei-li¹, XU Meng¹, JIAO Yan-mei¹, XU Xiao-ning², CHEN De-xi¹, WU Hao²

1. HIV/AIDS & STD Laboratory, Beijing Youan Hospital, Capital Medical University;2. Department of Infections Diseases, Beijing Youan Hospital, Capital Medical University

Received:2009-06-16 Revised:1900-01-01 Online:2009-10-21 Published:2009-10-21

摘要/Abstract

摘要： 目的了解北京市部分男男同性恋(men who have sex with men,MSM)人群HIV-1的分子流行情况,监测该地区人群HIV毒株的最新流行情况。方法提取17例男男同性恋者HIV-1抗体阳性样本全血基因组DNA,直接进行巢式PCR扩增HIV env基因C2-V5区及gag基因P17-P24区,对PCR产物进行核苷酸序列测定和分析,确定基因亚型。结果 17例患者中,共存在CRF01-AE、B亚型和CRF07-BC 3种亚型,其中CRF01-AE 9例(52.94％),B亚型5例(29.41%),CRF07-BC 3例(17.65％)。结论小样本量的流行病学调查显示,北京地区部分男男同性恋人群中主要存在CRF01-AE、B和CRF07-BC 3种亚型;CRF01-AE已取代B亚型而成为北京市MSM人群中HIV主要流行亚型,CRF07-BC重组亚型在北京市MSM人群中增长迅速。

关键词: 男男同性恋, 人类免疫缺陷病毒1型, 亚型, 系统进化树分析

Abstract: Objective To investigate the molecular epidemiology of human immunodeficiency virus 1(HIV-1) infection among men who have sex with men(MSM) in Beijing and survey the up to date HIV-1 prevalence in this region. Methods Seventeen anti-HIV-1 positive samples of MSMs from 2007 2008 were collected, the genomic DNA were isolated directly from the whole blood, HIV-1 gag/env genes were amplified by nested PCR from the genomie of MSMs. The c2-v5 region of env gene and p17-p24 region of gag gene were sequenced for analyzing the subtype directly. Phylogenetic analyses were performed based on the nucleotide sequence data. Results There were three HIV-1 strains including CRF01-AE, B and CRF07-BC among seventeen MSMs in Beijing, genotyping showed that 52.94%(9/17) were CRF01-AE, 29.41%(5/17) were B and 17.65%(3/17) were CRF07-BC. Conclusion There are three HIV-1 subtypes among MSMs in Beijing from the small samples epidemiologic survey, and the CRF01-AE has replaced the B and became the most prevalent HIV-1 subtype in Beijing, and the CRF07-BC is increasing fast among MSMs in Beijing recently.

Key words: men who have sex with men, human immunodeficiency virus 1, subtype, phylogenetic analysis of gene

中图分类号:

R 512.91

刘志英;李海英;张彤;黄晓婕;魏飞力;徐萌;焦艳梅;徐小宁;陈德喜;吴昊. 北京市部分男男同性恋人群中HIV-1分子流行病学研究[J]. 首都医科大学学报, 2009, 30(5): 616-620.

LIU Zhi-ying;LI Hai-ying;ZHANG Tong;HUANG Xiao-jie;WEI Fei-li;XU Meng;JIAO Yan-mei;XU Xiao-ning;CHEN De-xi;WU Hao. Molecular Epidemiologic Study on Human Immunodeficiency Virus Type 1 Infection in Partial Men Who have Sex with Men(MSM) in Beijing[J]. Journal of Capital Medical University, 2009, 30(5): 616-620.

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北京市部分男男同性恋人群中HIV-1分子流行病学研究

Molecular Epidemiologic Study on Human Immunodeficiency Virus Type 1 Infection in Partial Men Who have Sex with Men(MSM) in Beijing

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