脱细胞血管基质的生物相容性研究

首都医科大学学报 ›› 2010, Vol. 31 ›› Issue (5): 649-652.

脱细胞血管基质的生物相容性研究

董建德¹, 张建^2*, 谷涌泉², 黄金洪¹, 高峰¹, 李春民³

1. 首都医科大学教学医院北京电力医院心胸血管外科;2. 首都医科大学宣武医院血管外科, 首都医科大学血管外科研究所;3. 首都医科大学附属北京复兴医院血管外科

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-10-21 发布日期:2010-10-21
通讯作者: 张建

Study for the Biocompatibility of Decellularized Vascular Scaffold

DONG Jian-de¹, ZHANG Jian^2*, GU Yong-quan², HUANG Jin-hong¹, GAO Feng¹, LI Chun-min³

1. Department of Cardiothoracic and Vascular Surgery, Beijing Electric Power Hospital, Capital Medical University;2. Department of Vascular Surgery, Xuanwu Hospital, Institute of Vascular Surgery, Capital Medical University;3. Department of Vascular Surgery, Beijing Fuxing Hospital, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2010-10-21 Published:2010-10-21
Contact: ZHANG Jian

摘要/Abstract

摘要：

目的评估Triton X-100和胰蛋白酶方法制备的猪脱细胞血管基质的生物相容性。方法分别采用体外细胞毒性试验（直接接触法和MTT法）检测脱细胞血管基质的细胞毒性，采用皮下植入试验来评估经脱细胞血管基质的免疫排斥反应。结果 L929细胞在预铺脱细胞血管基质的培养板内及不同浓度脱细胞基质浸提液内均生长良好，皮下植入试验显示2周后脱细胞血管基质的炎性反应明显减轻。结论经Triton X-100和胰蛋白酶方法制备的猪脱细胞血管基质具有良好的生物相容性。

关键词: 细胞外基质, 细胞相容性, 组织支架

Abstract:

Objective To evaluate the biocompatibility of decellularized vascular scaffold prepared with Triton X-100 and trypsin. Methods In vitro and in vivo tests were performed to evaluate the biocompatibility of the decellularized vascular scaffold. The in vitro tests included directcontact method and MTT assay, and the in vivo test was subcutaneous implant test.
Results L929 cells grew well on the glass slide covered with decellularized vascular scaffold and in the solution of decellularized vascular matrix. The patches implanted subcutaneously in SD mice only caused slight inflammatory reaction after 2 weeks. Conclusion Decellularized vascular matrices prepared with Triton X-100 and trypsin had a good biocompatibility.

Key words: extracellular matrix, biocompatibility, tissue scaffold

中图分类号:

R 543

董建德;张建;谷涌泉;黄金洪;高峰;李春民. 脱细胞血管基质的生物相容性研究[J]. 首都医科大学学报, 2010, 31(5): 649-652.

DONG Jian-de;ZHANG Jian;GU Yong-quan;HUANG Jin-hong;GAO Feng;LI Chun-min. Study for the Biocompatibility of Decellularized Vascular Scaffold[J]. Journal of Capital Medical University, 2010, 31(5): 649-652.

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