Abstract: Atrial fibrillation(AF) is a common cardiovascular disease associated with increased mortality and morbility. Catheter ablation of AF has emerged as a developing therapy to cure AF. However, scientific and normative strategy and technology of AF ablation were limited at the beginning of this century. With regard to this issue, the strategy and technology of AF ablation were systemic explored and some achievements were obtained in this project. ① A method of transseptal catheterization guided by fluoroscopy in right anterior oblique 45°was originally established; ② A method of pulmonary vein isolation and pulmonary vein mapping with single catheter was built; ③ This project provided the first evidence that circumferential pulmonary vein isolation was superior to segmental isolation; ④ It was proved that catheter ablation of valvular AF was safe and effective; ⑤ Some methods of catheter ablation of AF guided with 3 D mapping system in China were systemic established. This project deeply improved and facilitated the therapeutic and research level in the field of catheter ablation of AF.