膝骨关节炎滑膜不同部位基质金属蛋白酶3的表达及临床意义

doi:10.3969/j.issn.1006-7795.2013.03.017

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (3): 411-415.doi: 10.3969/j.issn.1006-7795.2013.03.017

膝骨关节炎滑膜不同部位基质金属蛋白酶3的表达及临床意义

李守龙¹, 艾依热提·买买提², 潘海涛¹, 曾峥¹

1. 首都医科大学附属北京天坛医院骨科,北京 100050;
2. 新疆油田公司明园职工医院外科,乌鲁木齐 830000

收稿日期:2013-01-15 出版日期:2013-06-21 发布日期:2013-06-17

Expression of matrix metalloproteinase-3 in different sites of osteoarthritis of knee synovial membrane and clinical significance

Li Shoulong¹, Maimaiti·Aiyireti², PAN Haitao¹, ZENG Zheng¹

1. Department of Orthopaedics,Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China;
2. Department of Surgery,Mingyuan Hospital of the Personnel,Affiliated to Xinjiang Petrolic Company,Urumqi 830000,China

Received:2013-01-15 Online:2013-06-21 Published:2013-06-17

摘要/Abstract

摘要：

目的研究膝骨关节炎(osteoarthritis of knee,KOA)滑膜不同部位基质金属蛋白酶3(matrix metalloproteinase-3,MMP-3)的表达强度,探索其与KOA发病机制的关系。方法选取48例KOA患者为研究对象,膝关节镜下滑膜清理术中分别取患侧膝关节内侧间室、髌上囊及外侧间室滑膜,并将患者按综合评分方法进行临床评分。行免疫组织化学染色,比较滑膜不同部位MMP-3的表达强度,并分析MMP-3表达强度与临床评分的相关性。结果 1内侧间室滑膜MMP-3表达强度为3.45±0.22,髌上囊滑膜为2.69±0.21,外侧间室为1.99±0.16,差异有统计学意义(P=0.000)。2内侧间室及髌上囊滑膜MMP-3表达强度与临床评分呈正相关关系,相关系数分别为r₁=0.894(P=0.000)、r₂=0.803(P=0.000);外侧间室滑膜MMP-3表达强度与临床评分无明显相关性r₃=0.485(P=0.057)。结论 MMP-3在KOA膝内侧间室滑膜的表达高于滑膜其他部位的表达,与临床表现为膝内侧症状重相符合;膝滑膜病变程度与临床评分呈正相关,为临床上有限切除滑膜病变缓解症状、改善功能提供了依据。

关键词: 膝骨关节炎, 滑膜, 基质金属蛋白酶3(MMP-3), 临床评分

Abstract:

Objective To determine the expression intensity of matrix metalloproteinase-3 (MMP-3) in different sites of osteoarthritis of knee synovial membrane and to explore its relationship with the pathogenesis of KOA. Methods Forty-eight KOA patients treated in our hospital were chosen as study subject. Knee synovial membrane specimens were excised separately from medial compartment,supra-patellar bursa and the lateral compartment during arthroscopy. Clinical scores of these patients were assessed by the comprehensive score system. Through immunohistochemical staining,MMP-3 positive expression intensity was compared among different sites of KOA synovial membrane. Moreover,the correlation between MMP-3 positive expression intensity and clinical scores was analyzed.Results 1MMP-3 positive expression intensity was 3.45±0.22 for medial compartment synovial membrane,2.69±0.21 for supra-patellar bursa,and 1.99±0.16 for lateral compartment,with statistically significant difference (P<0.001). 2MMP-3 positive expression intensity in synovial membrane of medial compartment and supra-patellar bursa presented a positive correlation with clinical scores,and the correlation coefficients were r₁=0.894(P<0.001) and r₂=0.803(P<0.001). There was no significant correlation between clinical scores and the positive expression intensity of MMP-3 in the lateral compartment with r₃=0.485(P=0.057).Conclusion MMP-3 expression intensity in synovial membrane of medial compartment was the highest of the three sites,which coincided with the manifestation that medial part of the involved knee joint suffers the most severe symptom. The disease degree of synovial membrane was positively correlated with clinical scores,providing a basis for improving the joint function and relieving the knee pain through limited resection of diseased synovial membrane by arthroscopy in clinical work.

Key words: osteoarthritis of knee(KOA), synovial membrane, matrix metalloproteinase 3(MMP-3), clinical score

中图分类号:

R 684.3

李守龙, 艾依热提·买买提, 潘海涛, 曾峥. 膝骨关节炎滑膜不同部位基质金属蛋白酶3的表达及临床意义[J]. 首都医科大学学报, 2013, 34(3): 411-415.

Li Shoulong, Maimaiti·Aiyireti, PAN Haitao, ZENG Zheng. Expression of matrix metalloproteinase-3 in different sites of osteoarthritis of knee synovial membrane and clinical significance[J]. Journal of Capital Medical University, 2013, 34(3): 411-415.

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膝骨关节炎滑膜不同部位基质金属蛋白酶3的表达及临床意义

Expression of matrix metalloproteinase-3 in different sites of osteoarthritis of knee synovial membrane and clinical significance

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