雷帕霉素对野百合碱诱导的大鼠肺动脉高压及肺血管重构的影响

doi:10.3969/j.issn.1006-7795.2013.04.015

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (4): 554-558.doi: 10.3969/j.issn.1006-7795.2013.04.015

雷帕霉素对野百合碱诱导的大鼠肺动脉高压及肺血管重构的影响

刘杰^1,2, 杜雨轩^1,2, 王望^1,2, 国桓^1,2, 王军^1,2, 王辰^2,3

1. 首都医科大学基础医学院生理学教研室, 北京 100069;
2. 北京市呼吸和肺循环疾病重点实验室, 北京 100020;
3. 卫生部北京医院, 北京 100730

收稿日期:2013-01-15 出版日期:2013-08-21 发布日期:2013-07-20
通讯作者: 王军, 王辰 E-mail:wang_jun@ccmu.edu.cn;cyh-birm@263.net
基金资助:
国家重点基础研究发展计划(973项目)(2009CB522107);国家自然科学基金(81070042);重大国际合作研究基金项目(30810103904);海外及港澳学者合作研究基金(81228001);北京呼吸与肺循环疾病重点实验室开放课题(RPCD201201,2011HXFB04);北京市教委科技发展计划项目(KM201310025004);宁夏自然科学基金(NZ1220);首都医科大学基础临床合作课题(12JL15)。

Effects of rapamycin on monocrotaline-induced pulmonary hypertension and pulmonary arterial remodeling in rats

LIU Jie^1,2, DU Yuxuan^1,2, WANG Wang^1,2, GUO Huan^1,2, WANG Jun^1,2, WANG Chen^2,3

1. Department of Physiology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China;
2. Beijing Key Laboratory of Respiratory and Pulmonary Circulation, Beijing 100020, China;
3. Beijing Hospital, Ministry of Health, Beijing 100730, China

Received:2013-01-15 Online:2013-08-21 Published:2013-07-20
Supported by:
This study was supported by A Grant from the Major State Basic Research Development Program of China(2009CB522107); National Natural Science Foundation of China(81070042); Major International Collaborative Research Foundation of China(30810103904); Overseas and HongKong and Macao Scholars of Collaborative Research Foundation of China(81228001); Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders(RPCD201201, 2011HXFB04); The Foundation of Scientific Research Common Program of Beijing Municipal Commission of Education(KM201310025004); Natural Science Foundation of Ningxia(NZ1220); Basis-clinical Research Co-operation Fund of Capital Medical University(12JL15).

摘要/Abstract

摘要：

目的探讨雷帕霉素对野百合碱诱导的大鼠肺动脉高压及肺血管重构的干预作用。方法雄性SD大鼠采用抽签法随机分为3组:对照组、野百合碱组和雷帕霉素干预组。采用经颈外静脉右心导管插管术检测右心室收缩压;计算右心室肥厚指数;采用HE染色,观察肺细小动脉结构改变情况。结果与对照组相比,野百合碱组大鼠体质量明显减轻,右心室收缩压显著升高,右心室肥厚指数明显增加,肺动脉重构显著。与野百合碱组相比,雷帕霉素干预组大鼠虽然体质量显著减轻,但右心室收缩压明显改善;右心室肥厚指数显著降低;肺动脉重构程度明显减轻。结论雷帕霉素有效干预了野百合碱诱导的大鼠肺动脉高压及肺血管重构的形成。提示雷帕霉素有可能作为临床上预防和治疗肺动脉高压的新药。

关键词: 雷帕霉素, 野百合碱, 肺动脉高压, 肺血管重构

Abstract:

Objective To investigate the effects of rapamycin on monocrotaline(MCT)-induced pulmonary hypertension(PH) and pulmonary arterial remodeling in rats. Methods Male Sprague-Dawley rats were divided into 3 groups: control group, MCT group and rapamycin group. The right ventricular systolic pressure(RVSP) was measured via the right jugular vein catheterization into the right ventricle. The index of right ventricular hypertrophy(RVHI) was calculated. HE staining was used to assess the distal vascular remodeling. Results The body weight of MCT group was significantly lower than that of the control group; The RVSP, RVHI and the remodeling of the small arteries were significantly higher than control group. Even the body weight of rats in rapamycin group was much lower than that of the control and MCT group, while the RVSP, RVHI and the remodeling of the small arteries were significantly improved in rapamycin group compared to MCT group. Conclusion These results suggested that rapamycin could attenuate the development of pulmonary hypertension and pulmonary vascular remodeling. The results provide the theoretical basis for rapamycin to be a potential drug for treating PH.

Key words: rapamycin, monocrotaline, pulmonary hypertension, pulmonary arterial remodeling

中图分类号:

R363

刘杰, 杜雨轩, 王望, 国桓, 王军, 王辰. 雷帕霉素对野百合碱诱导的大鼠肺动脉高压及肺血管重构的影响[J]. 首都医科大学学报, 2013, 34(4): 554-558.

LIU Jie, DU Yuxuan, WANG Wang, GUO Huan, WANG Jun, WANG Chen. Effects of rapamycin on monocrotaline-induced pulmonary hypertension and pulmonary arterial remodeling in rats[J]. Journal of Capital Medical University, 2013, 34(4): 554-558.

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雷帕霉素对野百合碱诱导的大鼠肺动脉高压及肺血管重构的影响

Effects of rapamycin on monocrotaline-induced pulmonary hypertension and pulmonary arterial remodeling in rats

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