NHERF3在肾癌组织中的表达变化及其意义

doi:10.3969/j.issn.1006-7795.2015.02.016

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (2): 251-254.doi: 10.3969/j.issn.1006-7795.2015.02.016

NHERF3在肾癌组织中的表达变化及其意义

法朋燕¹, 刘华¹, 郑君芳¹, 王磊², 代元平¹, 贺俊崎¹

1. 首都医科大学基础医学院生物化学与分子生物学系, 北京 100069;
2. 首都医科大学附属北京友谊医院泌尿外科, 北京 100069

收稿日期:2015-02-08 发布日期:2015-04-16
通讯作者: 贺俊崎 E-mail:jq_he@ccmu.edu.cn
基金资助:
国家自然科学基金(81372739),2013年首都医科大学基础-临床科研合作基金(13JL73)。

NHERF3 expression level and its significance in renal cell carcinoma

Fa Pengyan¹, Liu Hua¹, Zheng Junfang¹, Wang Lei², Dai Yuanping¹, He Junqi¹

1. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
2. Department of Urology Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China

Received:2015-02-08 Published:2015-04-16
Supported by:
This study was supported by National Natural Science Foundation of China(81372739), Basic-Clinical Scientific Research Cooperation Fund of Capital Medical University(13JL73).

摘要/Abstract

摘要： 目的研究NHERF3在肾癌及其癌旁组织中的表达变化,为揭示肾癌组织NHERF3表达变化的临床意义提供线索。方法分别采用免疫组织化学法、Western blotting和RT-PCR方法检测肾癌及其对应癌旁组织中NHERF3蛋白及mRNA的表达,比较二者的差异。结果在癌旁组织中,NHERF3蛋白有较丰富的表达,而在肾癌组织中NHERF3蛋白的表达十分微弱(P<0.01);癌旁组织NHERF3 mRNA表达水平亦明显高于肾癌组织(P<0.05),二者之间的差异均有统计学意义。结论肾癌组织中NHERF3蛋白及mRNA与癌旁组织比较均呈现异常低表达,表明NHERF3基因表达在转录水平受到明显抑制,提示与肾癌NHERF3蛋白缺乏相关的NHERF3功能缺失(loss of function)可能在肾癌发生、发展中起重要作用。

关键词: 肾癌, NHERF3, 基因表达

Abstract: Objective To analysis the difference of the expression level of NHERF3 between renal cell carcinoma and its adjacent tissues. Methods To detect the expression of NHERF3 protein by immunohistochemistry and Western blotting, mRNA by RT-PCR in renal cell carcinoma and their corresponding adjacent tissues. Results NHERF3 protein and mRNA level is significantly decreased in renal cell carcinoma when compared with that in adjacent tissues. Conclusion NHERF3 gene expression was significantly inhibited at the transcriptional level.

Key words: renal cell carcinoma, NHERF3, gene expression

中图分类号:

法朋燕, 刘华, 郑君芳, 王磊, 代元平, 贺俊崎. NHERF3在肾癌组织中的表达变化及其意义[J]. 首都医科大学学报, 2015, 36(2): 251-254.

Fa Pengyan, Liu Hua, Zheng Junfang, Wang Lei, Dai Yuanping, He Junqi. NHERF3 expression level and its significance in renal cell carcinoma[J]. Journal of Capital Medical University, 2015, 36(2): 251-254.

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NHERF3在肾癌组织中的表达变化及其意义

NHERF3 expression level and its significance in renal cell carcinoma

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