脑特异性血管抑制因子-1在宫颈鳞癌中的表达及其与人乳头瘤病毒感染的关系

doi:10.3969/j.issn.1006-7795.2018.04.017

首都医科大学学报 ›› 2018, Vol. 39 ›› Issue (4): 569-574.doi: 10.3969/j.issn.1006-7795.2018.04.017

脑特异性血管抑制因子-1在宫颈鳞癌中的表达及其与人乳头瘤病毒感染的关系

张俊俊^1,2, 王焱², 杜趁香³, 晋熙梦^1,2

1. 河南科技大学临床医学院, 河南洛阳 471003;
2. 河南科技大学第一附属医院妇科, 河南洛阳 471003;
3. 郑州大学人民医院产科, 郑州 450003

收稿日期:2018-01-16 出版日期:2018-07-21 发布日期:2018-07-21
通讯作者: 王焱 E-mail:doctoryanyan@126.com
基金资助:
洛阳市科技攻关项目基金（1201052A-2）。

Expression of brain-specific angiogenesis inhibitor-1 in cervical squamous cell carcinomas correlates with human papillomavirus infection

Zhang Junjun^1,2, Wang Yan², Du Chenxiang³, Jin Ximeng^1,2

1. College of Clinical Medicine of Henan University of Science and Technology, Luoyang 471003, Henan Province, China;
2. Department of Gynaecology of the First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province, China;
3. Department of Obstetric, People's Hospital of Zhengzhou University, Zhengzhou 450003, China

Received:2018-01-16 Online:2018-07-21 Published:2018-07-21
Supported by:
This study was supported by Science and Technology Tackling Project Foundation of Luoyang(1201052A-2).

摘要/Abstract

摘要： 目的观察脑特异性血管抑制因子-1（brain-specific angiogenesis inhibitor-1，BAI-1）在宫颈病变组织中的表达差异，分析其在宫颈鳞癌发生发展中存在的意义及与人乳头瘤病毒（human papillomavirus，HPV）感染的关系。方法分别通过SP法和PCR反向点杂交法对BAI-1和HPV在30例正常宫颈组织、61例宫颈上皮内瘤变（cervical intraepithelial neoplasia，CIN）组织（CIN Ⅰ27例，CIN Ⅱ~Ⅲ34例）以及54例宫颈鳞癌组织（Ⅰ~Ⅱ期34例，Ⅲ~Ⅳ期20例）中的表达和感染情况进行总结分析，从而探讨其与宫颈鳞癌发生发展的关系及与HPV感染的相关性。结果 BAI-1在不同宫颈组织中的阳性表达率分别为96.67%、74.07%、44.11%、18.52%，而HPV的阳性感染率为6.67%、37.04%、76.47%、100%。分析数据得出，随着宫颈病变的进展，BAI-1的阳性表达率逐渐在下降，而HPV的阳性感染率在上升，组间差异均有统计学意义（P<0.01）；且BAI-1与宫颈鳞癌的临床分期有关（χ²=7.219，P=0.007），与年龄、分化程度及有无淋巴结转移无关；在不同宫颈组织中BAI-1的阳性表达与HPV的阳性感染呈负相关（r_s=-0.486，P<0.01）。结论 BAI-1可能参与HPV感染导致的宫颈鳞癌的早期发生发展过程，为宫颈鳞癌的诊治及预后提供了新的方向。

关键词: 脑特异性血管抑制因子-1, 人乳头瘤病毒感染, 宫颈鳞癌

Abstract: Objective To test the expression of brain-specific angiogenesis inhibitor-1 (BAI-1) in cervical lesion, and analyze the significance in the development of cervical squamous cell carcinomas and the correlation with human papillomavirus (HPV) infection. Methods By SP method and PCR reverse dot hybridization to summarize and analyze the expression of BAI-1 and infection of HPV in 30 cases of normal cervical tissue, 61 cases of cervical intraepithelial neoplasia (CIN) (CIN Ⅰ 27 cases, CIN Ⅱ-Ⅲ 34 cases) and 54 cases of cervical squamous carcinoma tissues (Ⅰ-Ⅱ 34 cases, Ⅲ-IV 20 cases), discussing the correlation in the development of cervical squamous cell carcinoma and the correlation with HPV infection. Results The positive expression rates of BAI-1 in normal cervical tissue, CIN Ⅰ, CIN Ⅱ -Ⅲ and cervical squamous cell carcinoma tissues were 96.67%, 74.07%, 44.11% and 18.52%. And the positive infection rates of HPV were 6.67%, 37.04%, 76.47% and 100%. By analyzing the data, as the cervical lesions progressed, the positive expression rate of BAI-1 was falling gradually, and the positive infection rate of HPV was rising gradually, differences between groups were statistically significant (P<0.01). The positive expression of BAI-1 was only relevant with the clinical stage (χ²=7.219, P=0.007), and has nothing to do with age, histological grade and lymph node metastasis; the positive expression of BAI-1 was negatively correlated with the positive infection rate of HPV (r_s=-0.486, P<0.01) in different cervical tissues. Conclusion BAI-1 was may participate in the early lesion process of cervical cancer of HPV infection, to provide a new direction for diagnosis, treatment and prognosis of cervical squamous carcinoma.

Key words: brain-specific angiogenesis inhibitor-1, human papillomavirus infection, cervical squamous cell carcinomas

中图分类号:

R711.1

张俊俊, 王焱, 杜趁香, 晋熙梦. 脑特异性血管抑制因子-1在宫颈鳞癌中的表达及其与人乳头瘤病毒感染的关系[J]. 首都医科大学学报, 2018, 39(4): 569-574.

Zhang Junjun, Wang Yan, Du Chenxiang, Jin Ximeng. Expression of brain-specific angiogenesis inhibitor-1 in cervical squamous cell carcinomas correlates with human papillomavirus infection[J]. Journal of Capital Medical University, 2018, 39(4): 569-574.

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脑特异性血管抑制因子-1在宫颈鳞癌中的表达及其与人乳头瘤病毒感染的关系

Expression of brain-specific angiogenesis inhibitor-1 in cervical squamous cell carcinomas correlates with human papillomavirus infection

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