探究早期帕金森病Thy 1-SNCA模型小鼠运动学习障碍的时间特征

doi:10.3969/j.issn.1006-7795.2024.05.021

首都医科大学学报 ›› 2024, Vol. 45 ›› Issue (5): 881-890.doi: 10.3969/j.issn.1006-7795.2024.05.021

探究早期帕金森病Thy 1-SNCA模型小鼠运动学习障碍的时间特征

付文欣¹, 王铁², 杨汝宁²,曲明芹²,高歌²,杨慧², 贾军^1*

1.首都医科大学基础医学院生理学与病理生理学学系，北京 100069；2.首都医科大学基础医学院神经生物学学系，北京 100069

收稿日期:2024-04-02 出版日期:2024-10-21 发布日期:2024-10-18
通讯作者: 贾军 E-mail:jiajun@ccmu.edu.cn
基金资助:
国家自然科学基金项目（32271173, 81870994），北京市自然科学基金项目（7242214）。

Investigating the temporal characteristics of motor learning deficits in Thy 1-SNCA early Parkinson's disease model mice

Fu Wenxin¹, Wang Tie², Yang Runing², Qu Mingqin², Gao Ge², Yang Hui², Ja Jun^1*

1.Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China; 2. Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2024-04-02 Online:2024-10-21 Published:2024-10-18
Supported by:
This study was supported by National Natural Science Foundation of China (32271173, 81870994), Natural Science Foundation of Beijing(7242214).

摘要/Abstract

摘要： 目的本实验通过建立早期帕金森病（Parkinson's disease, PD）小鼠模型，以探究PD模型出现运动学习障碍的时间特征。方法选用6月龄 Thy 1-SNCA转基因（transgenic, TG）小鼠作为早期PD模型，与同龄野生型（wild type, WT）小鼠进行对比分析。通过旷场实验、爬杆实验和Y迷宫等行为学手段来评估小鼠的运动功能和空间工作记忆能力，并以经典的匀加速转棒实验作为运动学习的行为学范式，来评估其运动学习能力。结果 6月龄的Thy 1-SNCA TG小鼠在运动功能和空间记忆能力方面并未显示出异常，却呈现出明显的运动学习障碍。结论在早期PD模型小鼠上存在着特定的运动学习障碍，可作为早期PD的一个重要诊断指标；同时匀加速转棒测试可作为检测PD运动学习障碍的一种重要的行为学手段。

关键词: 帕金森病, 早期帕金森病模型, 运动学习障碍, 运动症状

Abstract: Objective In this experiment, a mouse model of early Parkinson's (PD) disease was established to explore the temporal characteristics of PD motor learning deficits. Methods Six-month-old Thy 1-SNCA transgenic mice were selected as an early PD model and compared with wild type (WT) mice for analysis. The mice's motor function and spatial working memory ability were assessed by experiments such as open field test, pole test, and Y-maze test, and the typical accelerating rotarod test was used as a behavioral paradigm for motor learning. Results The six-month-old Thy 1-SNCA transgenic mice did not show abnormalities in motor function and spatial working memory ability, but presented obvious characteristics of motor learning deficits. Conclusions Specific motor learning deficits exist on early PD model mice, which can be used as a detection indicator of early PD. And the typical accelerating rotarod test can be used as a key behavioral method for detecting motor learning deficits in PD.

Key words: Parkinson's disease, early Parkinson's disease model, motor learning deficits, motor symptoms

中图分类号:

R741.02

付文欣, 王铁, 杨汝宁, 曲明芹, 高歌, 杨慧, 贾军. 探究早期帕金森病Thy 1-SNCA模型小鼠运动学习障碍的时间特征[J]. 首都医科大学学报, 2024, 45(5): 881-890.

Fu Wenxin, Wang Tie, Yang Runing, Qu Mingqin, Gao Ge, Yang Hui, Ja Jun. Investigating the temporal characteristics of motor learning deficits in Thy 1-SNCA early Parkinson's disease model mice[J]. Journal of Capital Medical University, 2024, 45(5): 881-890.

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探究早期帕金森病Thy 1-SNCA模型小鼠运动学习障碍的时间特征

Investigating the temporal characteristics of motor learning deficits in Thy 1-SNCA early Parkinson's disease model mice

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