层流低切应力刺激对人脐静脉血管内皮细胞Toll样受体mRNA表达的影响

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (6): 771-773.

层流低切应力刺激对人脐静脉血管内皮细胞Toll样受体mRNA表达的影响

梁峰¹, 胡大一², 吴明营², 李田昌², 史旭波², 汤楚中², 王伯瑶³, 黄宁³, 吴琦³, 刘旭晴⁴

1. 北京市大兴区人民医院心脏内科;2. 首都医科大学附属北京同仁医院心脏诊疗中心;3. 四川大学华西医学中心;4. 北京市西城区平安医院

收稿日期:2005-05-08 修回日期:1900-01-01 出版日期:2006-12-24 发布日期:2006-12-24
通讯作者: 胡大一

The Changes of Toll-like Receptor mRNA Expression in Human Umbilical Vein Endothelial Cells after Lower Laminar Shear Stress

Liang Feng¹, Hu Dayi², Wu Mingying², Li Tianchang², Shi Xubo², Tang Chuzhong², Wang Boyao³, Huang Ning³, Wu Qi³, Liu Xuqing⁴

1. Department of Cardiology, Daxing District People's Hospital;2. Cardiovascular Center, Beijing Tongren Hospital, Capital University of Medical Sciences;3. West China Medical Center, Sichuan University;4. Beijing Xicheng District Pingan Hospital

Received:2005-05-08 Revised:1900-01-01 Online:2006-12-24 Published:2006-12-24

摘要/Abstract

摘要： 目的用RT-PCR和Northern杂交技术,评价层流低切应力刺激对人脐静脉血管内皮细胞Toll样受体(TLR-4)信号受体表达的影响.方法用未刺激和经42 μN/cm²处理1 h的脐静脉血管内皮细胞提取总RNA,采用RT-PCR和Northern杂交技术观察切应力刺激1 h人脐静脉血管内皮细胞TLR-2和TLR-4 mRNA的表达情况.结果 RT-PCR和Northern杂交均显示,层流低切应力刺激1 h后血管内皮细胞TLR-4表达增强,TLR-2几乎无变化.结论层流低切应力可引起人脐静脉内皮细胞TLR-4 mRNA表达增加,提示炎症信号受体TLR-4可能介导层流切应力诱导的血管内皮细胞某些效应基因的表达.

关键词: 人血管内皮细胞, 切应力, Toll样受体

Abstract: Objective By using the technology of RT-PCR and Northern hybridization,to assess the expression of TLR-2 and TLR-4 in human umbilical vein endothelial cells(HUVECs) after lower laminar shear stress.Methods Total RNA was isolated from HUVECs untreated and treated with 42 μN/cm² for 1 h.The technology of RT-PCR and Northern hybridization were used to determine the change of TLR-2 and TLR-4 mRNAs expression after 1 hour stimulation of laminar shear stress.Results The results of RT-PCR and Northern hybridization showed that the expression of TLR-4 in HUVECs was increased after 1 h shear stress.There was no change for TLR-2 after stimulation.Conclusion Lower laminar shear stress can induce TLR-4 mRNA expression.The experiment suggests that the inflammatory signal receptor TLR-4 would probably be involved in flow shear stress-induced effective gene expression in human vascular endothelial cells.

Key words: human umbilical vein endothelial cells, shear stress, Toll like receptor

中图分类号:

R541.4

梁峰;胡大一;吴明营;李田昌;史旭波;汤楚中;王伯瑶;黄宁;吴琦;刘旭晴. 层流低切应力刺激对人脐静脉血管内皮细胞Toll样受体mRNA表达的影响[J]. 首都医科大学学报, 2006, 27(6): 771-773.

Liang Feng;Hu Dayi;Wu Mingying;Li Tianchang;Shi Xubo;Tang Chuzhong;Wang Boyao;Huang Ning;Wu Qi;Liu Xuqing. The Changes of Toll-like Receptor mRNA Expression in Human Umbilical Vein Endothelial Cells after Lower Laminar Shear Stress[J]. Journal of Capital Medical University, 2006, 27(6): 771-773.

[1]	段楠, 黄辰炜, 逄璐, 李海霞. 糖化白蛋白调控肾损伤相关分子与Toll样受体信号通路及齐墩果酸对其干预作用的研究[J]. 首都医科大学学报, 2019, 40(4): 609-614.
[2]	李艳, 李波, 姚秀娟, 吕喆, 安云庆, 王炜, 黄克武, 孙英. Toll样受体11~13在肥大细胞上的表达[J]. 首都医科大学学报, 2013, 34(2): 251-257.
[3]	马瑞敏;张国军;康熙雄. Toll样受体与自身免疫性疾病关系的研究进展[J]. 首都医科大学学报, 2012, 33(2): 177-181.
[4]	张婷;王晓民. 揭示免疫应答的关键环节2011年诺贝尔生理学或医学奖简介[J]. 首都医科大学学报, 2011, 32(5): 710-713.
[5]	孙丽翠;王雅梅;闫豫东;张静宜;司杨;祁雅慧. 人VEGF基因杆状病毒表达载体的构建及生物学活性鉴定[J]. 首都医科大学学报, 2006, 27(5): 623-626.
[6]	梁峰;胡大一;王伯瑶;陈槐卿. 炎症信号受体TLR-4基因的克隆及真核表达[J]. 首都医科大学学报, 2004, 25(1): 40-43.